Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Janelia Fluor 549

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # 521204
View all F4/80 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549

Specificity

Detects mouse F4/80/EMR1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for F4/80 Antibody (521204) [Janelia Fluor® 549]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: F4/80

F4/80 antigen is a cell surface glycoprotein that is the murine homolog of human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) (1,2). Specifically, F4/80 (EMR1) is one of five members belonging to the adhesion G-coupled receptor (ADGRE) subfamily, also known as the epidermal growth factor seven transmembrane (EGF-TM7) receptors (1,2). F4/80 is expressed by the myeloid lineage including in eosinophils, monocytes, macrophages, and myeloid dendritic cells (1,2). The molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1).

Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).

References

1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7

2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15

Alternate Names

DD7A5-7, Gpf480, Ly71

Gene Symbol

Adgre1

Additional F4/80 Products

Product Documents for F4/80 Antibody (521204) [Janelia Fluor® 549]

Certificate of Analysis

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Product Specific Notices for F4/80 Antibody (521204) [Janelia Fluor® 549]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for F4/80 Antibody (521204) [Janelia Fluor® 549]

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Are the F4/80/EMR1 antibodies validated in Simple Western?

    A: No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.

  • Q: Does F4/80/EMR1 antibodies come in lyophilized form?

    A: Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975

  • Q: What is the theoretical molecular weight for F4/80/EMR1 antibodies?

    A: The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.

  • Q: Are the F4/80/EMR1 antibodies validated in Simple Western?

    A: No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.

  • Q: Does F4/80/EMR1 antibodies come in lyophilized form?

    A: Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975

  • Q: What is the theoretical molecular weight for F4/80/EMR1 antibodies?

    A: The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.

  • Q: Are the F4/80/EMR1 antibodies validated in Simple Western?

    A: No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.

  • Q: Does F4/80/EMR1 antibodies come in lyophilized form?

    A: Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975

  • Q: What is the theoretical molecular weight for F4/80/EMR1 antibodies?

    A: The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.

Showing  1 - 3 of 3 FAQs Showing All
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