Mouse ICAM-1/CD54 APC-conjugated Antibody

FAB796A has been discontinued. View all ICAM-1/CD54 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and recombinant mouse (rm) ICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, recombinant rat (rr) ICAM-1, rmMAdCAM-1, or rmVCAM-1.
  • Source
    Monoclonal Rat IgG2B Clone # 166623
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1
    Gln28-Asn485
    Accession # P13597
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ICAM‑1/CD54 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse ICAM‑1/CD54 APC‑conjugated Monoclonal Antibody (Catalog # FAB796A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ICAM-1/CD54
ICAM-1 (InterCellular Adhesion Molecule-1), also known as CD54, is an 85-93 kDa member of the Immunoglobulin supergene family of molecules. Mouse ICAM-1 is a 510 amino acid (aa) type I transmembrane glycoprotein that contains five C2-type Ig-like domains in a 458 aa extracellular domain (ECD) (aa 28-485). It is expressed constitutively by, or inducibly on, a very wide variety of cells, including neurons, dendritic cells, fibroblasts, skeletal muscle myocytes, keratinocytes, eosinophils, neutrophils, hepatocytes, monocytes and vascular endothelial cells. On the cell surface, it exists primarily as an "intermediate" between a homodimeric and monomeric state. The homodimeric pairing does not seem to offer a distinct advantage with respect to ligand binding. On the other hand, the degree of glycosylation has a marked impact on ICAM-1 ligand binding, with under-glycosylation associated with low affinity ligand binding. Ligands for ICAM-1 include LFA-1 ( alpha L beta 2), Mac-1 ( alpha M beta 2), CD11c/CD18, fibrinogen, hyaluronan and select rhinoviruses. Notably, ICAM-1 is recognized to be a potential coreceptor for HGF, acting in concert with c-Met during ligand binding. ICAM-1 is best known for its role in white cell trafficking (leukocyte binding to endothelium), and is also reported to play a key role in the establishment of CD8+ T cell memory, serving as a link between mature dendritic cells and naïve CD8+ T cells. Over its ECD, mouse ICAM-1 shares 78% and 54% aa sequence identity with rat and human ICAM-1 ECD, respectively. There is one isoform variant for mouse ICAM-1 listed in SwissProt that shows a 28 aa substitution for aa 1-34.
  • Long Name:
    Intercellular Adhesion Molecule 1
  • Entrez Gene IDs:
    3383 (Human); 15894 (Mouse); 25464 (Rat)
  • Alternate Names:
    BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
Related Research Areas

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