Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and recombinant mouse (rm) ICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, recombinant rat (rr) ICAM-1, rmMAdCAM-1, or rmVCAM-1.
Monoclonal Rat IgG2B Clone # 166623
Protein A or G purified from hybridoma culture supernatant
Detection of ICAM‑1/CD54 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse ICAM‑1/CD54 APC‑conjugated Monoclonal Antibody (Catalog # FAB796A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
ICAM-1 (InterCellular Adhesion Molecule-1), also known as CD54, is an
85-93 kDa member of the Immunoglobulin supergene family of molecules. Mouse ICAM-1 is a 510 amino acid (aa) type I
transmembrane glycoprotein that contains five C2-type Ig-like domains in a 458
aa extracellular domain (ECD) (aa 28-485).
It is expressed constitutively by, or inducibly on, a very wide variety of
cells, including neurons, dendritic cells, fibroblasts, skeletal muscle
myocytes, keratinocytes, eosinophils, neutrophils, hepatocytes, monocytes and
vascular endothelial cells. On the cell
surface, it exists primarily as an "intermediate" between a homodimeric and
monomeric state. The homodimeric pairing
does not seem to offer a distinct advantage with respect to ligand
binding. On the other hand, the degree
of glycosylation has a marked impact on ICAM-1 ligand binding, with under-glycosylation
associated with low affinity ligand binding.
Ligands for ICAM-1 include LFA-1 ( alpha L beta 2), Mac-1 ( alpha M beta 2), CD11c/CD18, fibrinogen,
hyaluronan and select rhinoviruses.
Notably, ICAM-1 is recognized to be a potential coreceptor for HGF,
acting in concert with c-Met during ligand binding. ICAM-1 is best known for its role in white
cell trafficking (leukocyte binding to endothelium), and is also reported to
play a key role in the establishment of CD8+ T cell memory, serving as a link
between mature dendritic cells and naïve CD8+ T cells. Over its ECD, mouse ICAM-1 shares 78% and 54%
aa sequence identity with rat and human ICAM-1 ECD, respectively. There is one isoform variant for mouse ICAM-1
listed in SwissProt that shows a 28 aa substitution for aa 1-34.
Intercellular Adhesion Molecule 1
Entrez Gene IDs:
3383 (Human); 15894 (Mouse); 25464 (Rat)
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
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