Rat IgG2B APC‑conjugated Isotype Control

(4 citations)   
  • Specificity
    Selected for use as a rat IgG2B isotype control for flow cytometry applications.
  • Source
    Monoclonal Rat IgG2B Clone # 141945
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat IgGControl by Flow Cytometry Mouse splenocytes treated with 1 μg/mL Concanavalin A for 3days were stained with Rat Anti-Mouse ICOS APC‑conjugated Monoclonal Antibody (Catalog #FAB168A,filled histogram) or Rat IgG APC-conjugated Isotype Control Antibody(Catalog # IC013A, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors
    Authors: SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk
    Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells.
    J. Immunol., 2012;189(3):1162-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  3. Targeted inhibition of T-cell factor activity promotes syndecan-2 expression and sensitization to doxorubicin in osteosarcoma cells and bone tumors in mice.
    J. Bone Miner. Res., 2012;27(10):2118-29.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  4. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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Rat IgG2B PerCP-conjugated Isotype Control

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Rat IgG2B Alexa Fluor® 594-conjugated Isotype Control

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Rat IgG2B Biotinylated Isotype Control

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Rat IgG2B Alexa Fluor® 647-conjugated Isotype Control

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Rat IgG2B Alexa Fluor® 488-conjugated Isotype Control

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Rat IgG2B Alexa Fluor® 750-conjugated Isotype Control

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Rat IgG2B Isotype Control

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