Q: Hello, I wonder if this product for flow cytometry needs a secondary antibody or not? If yes, how to choose the secondary?

A: This product is sold unconjugated and would need a secondary antibody. You will want to select a mouse IgG secondary to use with this primary. You can view all of our mouse IgG secondaries. You can filter this list down by selecting which conjugate you would prefer and which protein/target would work best for your experiment.

Mouse IgG Antibody Pair

Novus Biologicals | Catalog # NBP3-48426

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Sandwich ELISA

Assay Range

625-40000 pg/mL (example only; lot dependent)

Sensitivity

625 pg/mL (example only; lot dependent)

Reactivity

Mouse

View all Mouse IgG ELISA Kits »

Product Specifications

Description

Solid Phase sandwich ELISA for the quantitative determination of Mouse IgM using secondary antibodies for detection and capture.

Sample Volume Required

100 ul

Conjugate

Unconjugated

Scientific Data Images for Mouse IgG Antibody Pair

ELISA: Mouse IgG Antibody Pair [NBP3-48426] -

ELISA: Mouse IgG Antibody Pair [NBP3-48426] - Standard curve reference

Kit Contents for Mouse IgG Antibody Pair

Rabbit Monoclonal anti-Mouse IgM Capture Secondary Antibody
Rabbit Polyclonal anti-Mouse IgM Detection Secondary Antibody (HRP-conjugated)
Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Mouse IgG

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10E11 variants. Mouse IgG is one of the most common secondary antibodies used by researchers.

Additional Mouse IgG Products

Product Documents for Mouse IgG Antibody Pair

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse IgG Antibody Pair

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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