Mouse IL-17 ELISpot Development Module, 5 Plate

Name: Mouse IL-17 ELISpot Development Module, 5 Plate
Brand: R&D Systems
SKU: SEL421

Catalog #: SEL421
7 Citations Datasheet / COA / SDS

Discontinued Product

SEL421 has been discontinued.

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Product Details

Citations (7)

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Summary Product Datasheets Preparation and Storage Background

Mouse IL-17 ELISpot Development Module, 5 Plate Summary

Assay Type

Quantitative Sandwich ELISA Development Module

Format

96-well microplate, sold separately (see Other Reagents Required)

Assay Length

3 hours 35 mins to 4 hours 50 mins**

Sample Type

Whole Cells

Sufficient Materials

For five 96-well microplates

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

PRODUCT SUMMARY
Complete ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement. ELISpot Development Modules contain the basic components required to develop an ELISpot assay. They offer an economical alternative to buying separate antibodies.

ELISpot development modules are an alternative to ELISpot kits. A basic understanding of ELISpot assay development is required for the successful use of these reagents. Each investigator should optimize the coating conditions, the assay sensitivity, the type of enzyme and substrate, as well as the concentrations of the capture and detection antibodies to achieve desired results. The analyte-specific ELISpot Development Module and the ELISpot Blue Color Module contain the necessary components for analyte detection and visualization, respectively. These modules can be used together but are sold separately. Each module contains enough reagents for at least five 96-well microplates.

PRODUCT FEATURES

An economical alternative to ELISpot Kits
Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
Generic development protocols provide direction to start an optimization protocol
Customize the assay to your specific needs

MODULE CONTENTS

Mouse IL-17/IL-17A Capture Antibody
Mouse IL-17/IL-17A Biotinylated-conjugated Detection Antibody

OTHER REAGENTS REQUIRED

ELISpot Blue Color Module or equivalent (R&D Systems, Catalog # SEL002)
PBS - 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer - 0.05% Tween^® 20 in PBS
Blocking Buffer - 1% BSA, 5% Sucrose in PBS
Reagent Diluent - 1% BSA in PBS, pH 7.2 - 7.4, 0.2 µm filtered
2 °C – 8 °C refrigerator
37 °C CO₂ incubator
Positive Control - Use Recombinant Mouse IL-17/IL-17A or cells known to secrete Mouse IL-17/IL-17A
96-well plates - Nitrocellulose-bottom plates, PVDF-bottom Immunospot^® plates, or flat-bottom polystyrene Immulon^® ELISA plates
Multi-channel pipette, squirt bottle, manifold dispenser, or automated microplate washer
Dissection microscope or an automated ELISpot Reader
Deionized H₂O

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Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-17/IL-17A

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:

Interleukin 17

Entrez Gene IDs:

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names:

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Citations for Mouse IL-17 ELISpot Development Module, 5 Plate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Extracellular vesicles from human umbilical cord blood plasma modulate interleukin-2 signaling of T cells to ameliorate experimental autoimmune encephalomyelitis
Authors: S Kim, JY Maeng, SJ Hyun, HJ Sohn, SY Kim, CH Hong, TG Kim
Theranostics, 2020-04-06;10(11):5011-5028.
Species: Human
Sample Types: Cells
Pharmacological inhibition of ROR?t suppresses the Th17 pathway and alleviates arthritis in vivo
Authors: U Guendisch, J Weiss, F Ecoeur, JC Riker, K Kaupmann, J Kallen, S Hintermann, D Orain, J Dawson, A Billich, C Guntermann
PLoS ONE, 2017-11-20;12(11):e0188391.
Species: Rat
Sample Types: Whole Cells
Retinoic-acid-orphan-receptor-C inhibition suppresses Th17 cells and induces thymic aberrations
Authors: C Guntermann, A Piaia, ML Hamel, D Theil, T Rubic-Schn, A Del Rio-Es, L Dong, A Billich, K Kaupmann, J Dawson, K Hoegenauer, D Orain, S Hintermann, R Stringer, DD Patel, A Doelemeyer, M Deurinck, J Schümann
JCI Insight, 2017-03-09;2(5):e91127.
Species: Rat
Sample Types: Whole Cells
Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency.
Authors: Abdel Fattah E, Bhattacharya A, Herron A, Safdar Z, Eissa N
J Immunol, 2015-04-17;194(11):5407-16.
Species: Mouse
Sample Types: Whole Cells
The TLR7 ligand 9-benzyl-2-butoxy-8-hydroxy adenine inhibits IL-17 response by eliciting IL-10 and IL-10-inducing cytokines.
Authors: Vultaggio A, Nencini F, Pratesi S, Maggi L, Guarna A, Annunziato F, Romagnani S, Parronchi P, Maggi E
J. Immunol., 2011-03-09;186(8):4707-15.
Species: Mouse
Sample Types: Whole Cells
TIM-3: a novel regulatory molecule of alloimmune activation.
Authors: Boenisch O, D'Addio F, Watanabe T, Elyaman W, Magee CN, Yeung MY, Padera RF, Rodig SJ, Murayama T, Tanaka K, Yuan X, Ueno T, Jurisch A, Mfarrej B, Akiba H, Yagita H, Najafian N
J. Immunol., 2010-10-18;185(10):5806-19.
Species: Mouse
Sample Types: Whole Cells
Desiccating stress promotion of Th17 differentiation by ocular surface tissues through a dendritic cell-mediated pathway.
Authors: Zheng X, De Paiva CS, Li DQ, Farley WJ, Pflugfelder SC
Invest. Ophthalmol. Vis. Sci., 2010-02-03;51(6):3083-91.
Species: Mouse
Sample Types: Whole Cells