Mouse IL-18 R alpha /IL-1 R5 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1216F-100
FAB1216F-025

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Detection of IL‑18 R alpha /IL‑1 R5 in Mouse Splenocytes by Flow Cytometry.
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Citations (2)
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Mouse IL-18 R alpha /IL-1 R5 Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑18 R alpha /IL‑1 R5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-1 RI, rmIL-1 RII, recombinant human (rh) IL-1 R3, rhIL-1 R4, recombinant rat (rr) IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR is observed.
Source
Monoclonal Rat IgG1 Clone # 112614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha /IL‑1 R5
Ser19-Gly326
Accession # Q61098
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL‑18 Ra/IL‑1 R5 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of IL‑18 R alpha /IL‑1 R5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse IL-18 Ra/IL-1 R5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1216F, filled histogram) or isotype control antibody (Catalog # IC005F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-18 R alpha/IL-1 R5

IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.

Long Name
Interleukin 18 Receptor alpha
Entrez Gene IDs
8809 (Human); 16182 (Mouse)
Alternate Names
CD218a; CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra

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Citations for Mouse IL-18 R alpha /IL-1 R5 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. IL-18 acts in synergy with IL-7 to promote ex vivo expansion of T lymphoid progenitor cells.
    Authors: Gandhapudi S, Tan C, Marino J, Taylor A, Pack C, Gaikwad J, Van De Wiele C, Wren J, Teague T
    J Immunol, 2015-03-16;194(8):3820-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
    Authors: Freeman B, Meyer C, Slifka M
    J Virol, 2014-04-16;88(13):7474-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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