Mouse IL-22 R alpha 1 Antibody

Catalog # Availability Size / Price Qty
MAB42941
MAB42941-SP
Detection of IL‑22 R alpha 1 in Hepa 1‑6 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (9)
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Mouse IL-22 R alpha 1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-22 R alpha 1 in direct ELISAs. In direct ELISAs, 25% cross-reactivity with recombinant human (rh) IL‑22 R alpha 1 is observed and no cross-reactivity with rhIL-20 R alpha, recombinant mouse (rm) IL-20 R alpha, rhIL-22BP, or rmIL-22BP is observed.
Source
Monoclonal Rat IgG2A Clone # 496514
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-22 R alpha 1
Thr18-Ala228
Accession # Q80XZ4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Hepa 1‑6 mouse hepatoma cell line and C2C12 mouse myoblast cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL‑22 Ra1 antibody in Hepa 1‑6 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑22 R alpha 1 in Hepa 1‑6 Mouse Cell Line by Flow Cytometry. Hepa 1‑6 mouse hepatoma cell line was stained with Rat Anti-Mouse IL‑22 Ra1 Monoclonal Antibody (Catalog # MAB42941, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

Flow Cytometry View Larger

Detection of IL‑22 R alpha 1 in C2C12 cells by Flow Cytometry. C2C12 cells were stained with Rat Anti-Mouse IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB42941, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22 R alpha 1

The IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 330 aa cytoplasmic domain (1). Within the ECD, mouse IL-22 R alpha 1 shares 78%, 78%, and 94% aa sequence identity with canine, human, and rat IL-22 R alpha 1, respectively. It shares 20%‑26% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22, and -24 receptors (2). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (3, 4), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (5, 6). In both cases, IL‑22 R alpha 1 functions as the high affinity ligand binding subunit, and subsequent association with IL-10 R beta or IL-20 R beta serves to stabilize the complex (3, 6‑9). IL‑22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but association with IL-10 R beta or IL-20 R beta is required for signal transduction (3, 7). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (8, 10). Even though it is a receptor for interleukins, IL‑22 R alpha 1 is not expressed on hematopoietic cells (7, 11, 12). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (7, 11‑14). IL‑22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (4, 12, 14, 15). IL‑22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (4, 15).

References
  1. Tachiiri, A. et al. (2003) Genes Immun. 4:153.
  2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. 
  3. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335. 
  4. Boniface, K. et al. (2005) J. Immunol. 174:3695. 
  5. Dumoutier, L. et al. (2001) J. Immunol. 167:3545. 
  6. Wang, M. et al. (2002) J. Biol. Chem. 277:7341. 
  7. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725. 
  8. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  9. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099
  10. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  12. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
  13. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
  14. Wolk, K. et al. (2004) Immunity 21:241.
  15. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
Long Name
Interleukin 22 Receptor
Entrez Gene IDs
58985 (Human); 230828 (Mouse)
Alternate Names
CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11

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Citations for Mouse IL-22 R alpha 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models
    Authors: SW Lu, HC Pan, YH Hsu, KC Chang, LW Wu, WY Chen, MS Chang
    Nat Commun, 2020;11(1):4611.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Production of Interleukin-20 cytokines limits bacterial clearance and lung inflammation during infection by Streptococcus pneumoniae
    Authors: F Madouri, O Barada, G Kervoaze, F Trottein, M Pichavant, P Gosset
    EBioMedicine, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Interleukin-20 targets podocytes and is upregulated in experimental murine diabetic nephropathy
    Authors: YH Hsu, HH Li, JM Sung, WY Chen, YC Hou, YH Weng, WT Lai, CH Wu, MS Chang
    Exp. Mol. Med., 2017;49(3):e310.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
    Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
    Mucosal Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice
    Nat Commun, 2016;7(0):13896.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Endogenous IL-22 plays a dual role in arthritis: regulation of established arthritis via IFN-gamma responses.
    Authors: Justa S, Zhou X, Sarkar S
    PLoS ONE, 2014;9(3):e93279.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection.
    Authors: Kumar P, Thakar M, Ouyang W, Malarkannan S
    Mucosal Immunol, 2013;6(1):69-82.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. IL-23-Mediated Epidermal Hyperplasia Is Dependent on IL-6.
    Authors: Lindroos J, Svensson L, Norsgaard H
    J. Invest. Dermatol., 2011;131(5):1110-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing.
    Authors: Li Z, Burns AR, Miller SB, Smith CW
    FASEB J., 2011;25(8):2659-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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