Mouse IL-22 R alpha 1 Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-22 R alpha 1 in direct ELISAs. In direct ELISAs, 25% cross-reactivity with recombinant human (rh) IL‑22 R alpha 1 is observed and no cross-reactivity with rhIL-20 R alpha, recombinant mouse (rm) IL-20 R alpha, rhIL-22BP, or rmIL-22BP is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 496514
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-22 R alpha 1
    Thr18-Ala228
    Accession # Q80XZ4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑22 R alpha 1 in Hepa 1‑6 Mouse Cell Line by Flow Cytometry. Hepa 1‑6 mouse hepatoma cell line was stained with Rat Anti-Mouse IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB42941, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
Immunocytochemistry
IL‑22 R alpha 1 in Hepa 1‑6 Mouse Cell Line. IL‑22 R alpha 1 was detected in immersion fixed Hepa 1‑6 mouse hepatoma cell line using Rat Anti-Mouse IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB42941) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-22 R alpha 1

The IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 330 aa cytoplasmic domain (1). Within the ECD, mouse IL-22 R alpha 1 shares 78%, 78%, and 94% aa sequence identity with canine, human, and rat IL-22 R alpha 1, respectively. It shares 20%‑26% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22, and -24 receptors (2). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (3, 4), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (5, 6). In both cases, IL‑22 R alpha 1 functions as the high affinity ligand binding subunit, and subsequent association with IL-10 R beta or IL-20 R beta serves to stabilize the complex (3, 6‑9). IL‑22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but association with IL-10 R beta or IL-20 R beta is required for signal transduction (3, 7). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (8, 10). Even though it is a receptor for interleukins, IL‑22 R alpha 1 is not expressed on hematopoietic cells (7, 11, 12). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (7, 11‑14). IL‑22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (4, 12, 14, 15). IL‑22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (4, 15).

  • References:
    1. Tachiiri, A. et al. (2003) Genes Immun. 4:153.
    2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. 
    3. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335. 
    4. Boniface, K. et al. (2005) J. Immunol. 174:3695. 
    5. Dumoutier, L. et al. (2001) J. Immunol. 167:3545. 
    6. Wang, M. et al. (2002) J. Biol. Chem. 277:7341. 
    7. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725. 
    8. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
    9. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099
    10. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
    11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
    12. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
    13. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
    14. Wolk, K. et al. (2004) Immunity 21:241.
    15. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
  • Long Name:
    Interleukin 22 Receptor
  • Entrez Gene IDs:
    58985 (Human); 230828 (Mouse)
  • Alternate Names:
    CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Interleukin-20 targets podocytes and is upregulated in experimental murine diabetic nephropathy
    Authors: YH Hsu, HH Li, JM Sung, WY Chen, YC Hou, YH Weng, WT Lai, CH Wu, MS Chang
    Exp. Mol. Med., 2017;49(3):e310.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fresh
  2. Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
    Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
    Mucosal Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice
    Nat Commun, 2016;7(0):13896.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Endogenous IL-22 plays a dual role in arthritis: regulation of established arthritis via IFN-gamma responses.
    Authors: Justa S, Zhou X, Sarkar S
    PLoS ONE, 2014;9(3):e93279.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection.
    Authors: Kumar P, Thakar M, Ouyang W, Malarkannan S
    Mucosal Immunol, 2013;6(1):69-82.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing.
    Authors: Li Z, Burns AR, Miller SB, Smith CW
    FASEB J., 2011;25(8):2659-68.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. IL-23-Mediated Epidermal Hyperplasia Is Dependent on IL-6.
    Authors: Lindroos J, Svensson L, Norsgaard H
    J. Invest. Dermatol., 2011;131(5):1110-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
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