Key Product Details

Species Reactivity

Rat

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 54447
View all IgG2A Isotype Controls »

Product Specifications

Immunogen

KLH

Specificity

Selected for use as rat IgG2A isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta ), rhBLC/BCA-1, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta 8-1, recombinant viral (rv) CMV UL146, rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rhEotaxin-3, rmEotaxin 2, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin -1, rhLymphytactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV Type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rhMIP-1 delta, rmMIP-2, rhMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 alpha, rmMIP-3 beta, rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTarc, rmTARC, rmTCA-3, rhTeck, or rmTeck.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat IgG2A Isotype Control

Detection of Rat IgG Isotype Control by Flow Cytometry

Detection of Rat IgG Control by Flow Cytometry

B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405, filled histogram) or Rat IgG Isotype Control Antibody (Catalog #MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog F0105B).

Detection of Mouse IgG2a by Functional

Detection of Mouse IgG2a by Functional

CCL17-mediated chemotaxis is inhibited in a dose-dependent manner by B202 and B225.EL4 cells were incubated with mouse CCL17 at 2/mL in the presence and absence of (A) B202, (B) B225 (C) CNTO 5516 which is an irrelevant rat:mouse chimeric antibody having a mouse IgG1 Fc or (D) MAB529 (rat antibody) which is a commercial antibody that neutralizes CCL17. Assay was repeated twice with five replicates per sample each time. Data are expressed as chemotactic index (CI = Average RLU from stimulated wells/average RLU from medium control; medium control = CI of 1). Antibody dose used for each sample is indicated along the x axis. Composite CI results from both experiments are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24339934), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Rat IgG2A Isotype Control

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.

Reviewed Applications

Read 6 reviews rated 4.5 using MAB006 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Rat IgG2A Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Rat IgG2A Isotype Control

For research use only

Citations for Rat IgG2A Isotype Control

Customer Reviews for Rat IgG2A Isotype Control (6)

4.5 out of 5
6 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Rat IgG2A Isotype Control?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 of 6 reviews Showing All
Filter By:
Clear Filters X
  • Rat IgG2A Isotype Control
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HMVEC human microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 11/13/2024
    Rat IgG2A Isotype Control MAB006
  • Rat IgG2A Isotype Control
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 07/25/2023
    Rat IgG2A Isotype Control MAB006
  • Rat IgG2A Isotype Control
    Name: Pinar Aksoy
    Application: Flow Cytometry
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 12/08/2022
    Pink is the isotype control stained sample and blue is the MSLN stained cells. Both antibodies were at 0.5 mg/mL, and used at 1:100 dilution. The secondary antibody was Rat F(ab)2 IgG (H+L) PE-conjugated antibody.
    Rat IgG2A Isotype Control MAB006
  • Rat IgG2A Isotype Control
    Name: Lu Bai
    Application: Flow Cytometry
    Sample Tested: bone marrow
    Species: Rat
    Verified Customer | Posted 05/06/2022
  • Rat IgG2A Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Cell culture supernatant
    Species: Human
    Verified Customer | Posted 05/01/2020
    Rat IgG2A Isotype Control MAB006
  • Rat IgG2A Isotype Control
    Name: Anonymous
    Verified Customer | Posted 06/13/2017
    Isotypr control for ICC
    Rat IgG2A Isotype Control MAB006

There are no reviews that match your criteria.

Showing  1 - 5 of 6 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Isotype Controls