Mouse Integrin  alpha 2/CD49b APC-conjugated Antibody

FAB1740A has been discontinued. View all Integrin alpha 2/CD49b products.
(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Integrin alpha 2/CD49b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 3, alpha 4, alpha 5, alpha E, or alpha V is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 235033
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b
    Tyr27-Thr1129
    Accession # Q62469
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Integrin  alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 PE‑conjugated Monoclonal Antibody (Catalog # FAB8319P) and either (A) Rat Anti-Mouse Integrin  alpha 2/CD49b APC‑conjugated Monoclonal Antibody (Catalog # FAB1740A) or (B) Rat IgG2B Allophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 2/CD49b
The Integrin alpha 2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with beta 1 Integrin. The alpha 2 beta 1 Integrin is a collagen receptor that binds expressing cells to collagen and regulates collagen and collagenase secretion. Notably, this subunit has an "I" domain that is resistant to proteolysis. Over amino acids
(aa) 27-1129, mouse Integrin alpha 2 shares 80% aa sequence identity with human Integrin alpha 2.
  • Entrez Gene IDs:
    3673 (Human); 16398 (Mouse)
  • Alternate Names:
    alpha-2 subunit; CD49b antigen; CD49b; Integrin alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITGA2; VLA 2; VLA-2 alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Differential IL-23 requirement for IL-22 and IL-17A production during innate immunity against Salmonella enterica serovar Enteritidis.
    Authors: Siegemund S, Schutze N, Schulz S, Wolk K, Nasilowska K, Straubinger RK, Sabat R, Alber G
    Int. Immunol., 2009;21(5):555-65.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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