Mouse Integrin  alpha 4/CD49d PE-conjugated Antibody

FAB2450P has been discontinued. View all Integrin alpha 4/CD49d products.
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Integrin alpha 4/CD49d in flow cytometry.
  • Source
    Monoclonal Rat IgG2A Clone # 265329
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 4/CD49d
    Tyr34-Phe976
    Accession # Q792F9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Integrin  alpha 4/CD49d in B16-F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin  alpha 4/CD49d PE‑conjugated Monoclonal Antibody (Catalog # FAB2450P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 4/CD49d

Integrin alpha 4, also known as CD49d and VLA sunbunit alpha, associates with either Integrin beta 1 or beta 7 subunits to form heterodimers that serve as receptors for fibronectin and VCAM-1. Integrin alpha 4 beta 1 is expressed on peripheral lymphocytes, thymocytes, and monocytes, while Integrin alpha 4 beta 7 is expressed on peripheral lymphocytes and a small subset of thymocytes. On T cells, the alpha 4 subunit may contribute to cytotoxic activity. Over amino acids 34-976, mouse Integrin alpha 4 shares 85% amino acid sequence identity to human Integrin alpha 4.

  • Entrez Gene IDs:
    3676 (Human); 16401 (Mouse)
  • Alternate Names:
    269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4
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