Mouse Integrin alpha 4/CD49d PE‑conjugated Antibody

Name: Mouse Integrin alpha 4/CD49d PE‑conjugated Antibody
Brand: R&D Systems
SKU: FAB2450P

R&D Systems | Catalog # FAB2450P

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Product Documents

Discontinued Product

FAB2450P has been discontinued. View all Integrin alpha 4/CD49d products.

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG_2A Clone # 265329

View all Integrin alpha 4/CD49d Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 4/CD49d
Tyr34-Phe976
Accession # Q792F9

Specificity

Detects mouse Integrin alpha 4/CD49d in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse Integrin alpha 4/CD49d PE‑conjugated Antibody

Detection of Integrin alpha 4/CD49d in B16-F1 Mouse Cell Line by Flow Cytometry.

B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin a4/CD49d PE-conjugated Monoclonal Antibody (Catalog # FAB2450P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Integrin alpha 4/CD49d PE‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: B16‑F1 mouse melanoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin alpha 4/CD49d

Integrin alpha 4, also known as CD49d and VLA sunbunit alpha, associates with either Integrin beta 1 or beta 7 subunits to form heterodimers that serve as receptors for fibronectin and VCAM-1. Integrin alpha 4 beta 1 is expressed on peripheral lymphocytes, thymocytes, and monocytes, while Integrin alpha 4 beta 7 is expressed on peripheral lymphocytes and a small subset of thymocytes. On T cells, the alpha 4 subunit may contribute to cytotoxic activity. Over amino acids 34-976, mouse Integrin alpha 4 shares 85% amino acid sequence identity to human Integrin alpha 4.