Mouse MGL1/2 (CD301a/b) PE-conjugated Antibody

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Detection of MGL1/2 (CD301a/b) in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (5)
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Mouse MGL1/2 (CD301a/b) PE-conjugated Antibody Summary

Species Reactivity
Detects mouse MGL1 (CD301a) and MGL2 (CD301b) in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse MGL1/2 (CD301a/b)
Accession # AAH14811
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MGL1/2 (CD301a/b) antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of MGL1/2 (CD301a/b) in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse MGL1/2 (CD301a/b) PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB4297P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MGL1/2 (CD301a/b)

Mouse MGL1 (macrophage galactose N-acetyl-galactosamine (GalNAc) specific Lectin 1, CD301a), also called ASGP-BP (asialoglycoprotein binding protein), is a 38 kDa type II transmembrane glycoprotein of the C-type lectin family (1). Two MGL proteins are encoded by separate genes in the mouse, but share 91% amino acid (aa) identity in the extracellular domain (ECD) (2). Only one MGL occurs in human and rat, and this is more structurally similar to mouse MGL1 than MGL2. However, mouse MGL1 binds Lewis X, in contrast to human MGL and mouse MGL2 which both bind specifically to terminal GalNAc residues (2). Lewis X is a trisaccharide commonly found on leukocytes and some tumor cells. Both mouse MGL proteins are expressed on immature dendritic cells. Mouse MGL1 and MGL2 are markers for connective tissue macrophages of a type termed alternately activated macrophages. These macrophages are induced by IL-4 that is produced during Th2-mediated inflammatory responses to parasitic infections or allergic airway inflammation (3, 4). Quantitative RT-PCR after helminth infection shows a peak of MGL1 expression at 7 days, while MGL2 shows increasing expression for at least 29 days (3). This, and data from MGL1 knockout mice (5), indicates that MGL1 is critical during the formation of granulation tissue, with MGL2 remaining involved during chronic infection. Mouse MGL1 is synthesized with an N-terminal 35 aa cytoplasmic region, a 21 aa transmembrane segment and a 248 aa ECD. The ECD contains one 129 aa carbohydrate recognition domain (CRD) that shows 78% and 63% aa identity with rat and human MGL, respectively.

  1. Sato, M. et al. (1992) J. Biochem. 111:331.
  2. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
  3. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
  4. Sato, K. et al. (2005) Int. Immunol. 17:559.
  5. Sato, K. et al. (2005) Blood 106:207.
Long Name
Macrophage Galactose-type C-lectin 1 and 2
Alternate Names
CD8; Leu2; MGL1/2; p32

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Citations for Mouse MGL1/2 (CD301a/b) PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. IL-4/IL-13 polarization of macrophages enhances Ebola virus glycoprotein-dependent infection
    Authors: KJ Rogers, B Brunton, L Mallinger, D Bohan, KM Sevcik, J Chen, N Ruggio, W Maury
    PLoS Negl Trop Dis, 2019-12-11;13(12):e0007819.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CCR2(-) and CCR2(+) corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion
    Authors: J Liu, Y Xue, D Dong, C Xiao, C Lin, H Wang, F Song, T Fu, Z Wang, J Chen, H Pan, Y Li, D Cai, Z Li
    Mucosal Immunol, 2017-01-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Akt2 deficiency protects from acute lung injury via alternative macrophage activation and miR-146a induction in mice.
    Authors: Vergadi, Eleni, Vaporidi, Katerina, Theodorakis, Emmanuel, Doxaki, Christin, Lagoudaki, Eleni, Ieronymaki, Elefther, Alexaki, Vassilia, Helms, Mike, Kondili, Eumorfia, Soennichsen, Birte, Stathopoulos, Efstathi, Margioris, Andrew N, Georgopoulos, Dimitrio, Tsatsanis, Christos
    J Immunol, 2013-11-25;192(1):394-406.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Obesity alters adipose tissue macrophage iron content and tissue iron distribution.
    Authors: Orr J, Kennedy A, Anderson-Baucum E, Webb C, Fordahl S, Erikson K, Zhang Y, Etzerodt A, Moestrup S, Hasty A
    Diabetes, 2013-10-15;63(2):421-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Tailoring the immune response by targeting C-type lectin receptors on alveolar macrophages using “pathogen-like” amphiphilic polyanhydride nanoparticles
    Authors: Ana V. Chavez-Santoscoy, Rajarshi Roychoudhury, Nicola L.B. Pohl, Michael J. Wannemuehler, Balaji Narasimhan, Amanda E. Ramer-Tait


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