Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit

R&D Systems | Catalog # MPTX30

R&D Systems
Discontinued Product
MPTX30 has been discontinued. View all Pentraxin 3/TSG-14 products.

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)

Sensitivity

0.02 ng/mL

Assay Range

0.23-15 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
View all Pentraxin 3/TSG-14 ELISA Kits »

Product Summary for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit

The Quantikine Mouse Pentraxin 3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse PTX3 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse PTX3 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PTX3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PTX3.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse PTX3

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 33 34 34
Mean (ng/mL) 0.54 2.09 6.86 0.63 2.28 7.18
Standard Deviation 0.01 0.05 0.20 0.07 0.16 0.41
CV% 1.9 2.4 2.9 11.1 7.0 5.7

Recovery for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit

The recovery of mouse PTX3 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 98-119
EDTA Plasma (n=4) 98 90-110
Heparin Plasma (n=4) 95 81-100
Serum (n=4) 102 90-117

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse PTX3 in each matrix were diluted with Calibrator Diluent and assayed.

Mouse Pentraxin 3/TSG-14 ELISA Linearity

Scientific Data Images for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit

Mouse Pentraxin 3/TSG-14 ELISA Standard Curve

Mouse Pentraxin 3/TSG-14 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Long Name

Tumor Necrosis Factor Stimulated Gene 14

Alternate Names

PTX3, TSG-14, TSG14

Entrez Gene IDs

5806 (Human); 19288 (Mouse)

Gene Symbol

PTX3

Additional Pentraxin 3/TSG-14 Products

Product Documents for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit

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Protocols

View specific protocols for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit (MPTX30):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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