Mouse Plexin A1 APC-conjugated Antibody

FAB4309A has been discontinued. View all Plexin A1 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Plexin A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 10% cross‑reactivity with recombinant mouse (rm) Plexin A3 is observed and no cross-reactivity with rmPlexin A2, recombinant human (rh) Plexin B1, rhPlexin C1, or rhPlexin D1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 408305
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Plexin A1
    Leu49-Gly165
    Accession # P70206
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Plexin A1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Plexin A1 APC‑conjugated Monoclonal Antibody (Catalog # FAB4309A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Plexin A1

Plexin A1 is an 1894 amino acid, 200 kDa type I transmembrane protein that is a member of the Plexin family of semaphorin signal transducers. Plexin-A1 binds the transmembrane Sema6D directly and binds the Class 3 secreted semaphorins indirectly via neuropilin-1 and 2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. In the region used as an immunogen, mouse and human Plexin A1 share 97% amino acid identity.

  • Entrez Gene IDs:
    5361 (Human); 18844 (Mouse)
  • Alternate Names:
    NOV; NOVP; Plexin A1; PLEXIN-A1; PLXN1; PLXNA1
Related Research Areas

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