Mouse Plexin A1 APC‑conjugated Antibody

R&D Systems | Catalog # FAB4309A

R&D Systems
Discontinued Product
FAB4309A has been discontinued. View all Plexin A1 products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 408305
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Product Specifications

Immunogen

E. coli-derived recombinant mouse Plexin A1
Leu49-Gly165
Accession # P70206

Specificity

Detects mouse Plexin A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 10% cross‑reactivity with recombinant mouse (rm) Plexin A3 is observed and no cross-reactivity with rmPlexin A2, recombinant human (rh) Plexin B1, rhPlexin C1, or rhPlexin D1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Plexin A1 APC‑conjugated Antibody

Detection of Plexin A1 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Plexin A1 in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Plexin A1 APC-conjugated Monoclonal Antibody (Catalog # FAB4309A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Plexin A1 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: bEnd.3 mouse endothelioma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Plexin A1

Plexin A1 is an 1894 amino acid, 200 kDa type I transmembrane protein that is a member of the Plexin family of semaphorin signal transducers. Plexin-A1 binds the transmembrane Sema6D directly and binds the Class 3 secreted semaphorins indirectly via neuropilin-1 and 2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. In the region used as an immunogen, mouse and human Plexin A1 share 97% amino acid identity.

Alternate Names

NOVP, PLXN1, PLXNA1

Entrez Gene IDs

5361 (Human); 18844 (Mouse)

Gene Symbol

PLXNA1

UniProt

Additional Plexin A1 Products

Product Documents for Mouse Plexin A1 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Plexin A1 APC‑conjugated Antibody

For research use only

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