Detects mouse Plexin A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 10% cross‑reactivity with recombinant mouse (rm) Plexin A3 is observed and no cross-reactivity with rmPlexin A2, recombinant human (rh) Plexin B1, rhPlexin C1, or rhPlexin D1 is observed.
Monoclonal Rat IgG2A Clone # 408305
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Plexin A1 Leu49-Gly165 Accession # P70206
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Plexin A1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Plexin A1 APC‑conjugated Monoclonal Antibody (Catalog # FAB4309A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Plexin A1
Plexin A1 is an 1894 amino acid, 200 kDa type I transmembrane protein that is a member of the Plexin family of semaphorin signal transducers. Plexin-A1 binds the transmembrane Sema6D directly and binds the Class 3 secreted semaphorins indirectly via neuropilin-1 and 2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. In the region used as an immunogen, mouse and human Plexin A1 share 97% amino acid identity.
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