Mouse Rae-1 Pan Specific PerCP-conjugated Antibody

FAB17582C has been discontinued. View all Rae-1 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Rae‑1 in flow cytometry. It recognizes Rae-1 alpha, beta, δ, gamma and epsilon.
  • Source
    Monoclonal Rat IgG2A Clone # 186107
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
    Leu29-Ser227
    Accession # Q9JI58
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae‑1 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB17582C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Rae-1
Rae-1 alpha, beta, gamma, δ and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.
  • Long Name:
    Retinoic Acid Early Transcript 1
  • Entrez Gene IDs:
    8480 (Human); 19368 (Mouse)
  • Alternate Names:
    Rae1; Rae-1
Related Research Areas

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