Mouse/Rat PRAT4B Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # AF5015AFP488

R&D Systems
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG
View all PRAT4B Primary Antibodies »

Product Specifications

Specificity

Detects mouse and rat PRAT4B in Western blots.  In direct ELISAs, less than 5% cross-reactivity with recombinant human PRAT4B and recombinant mouse PRAT4A is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse/Rat PRAT4B Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PRAT4B

PRAT4B (protein associated with TLR4-B; also Cnpy4, protein canopy homolog 4) is a member of the canopy family of proteins. It is expressed by diverse cell types, including macrophages, fibroblasts and B cells. PRAT4B appears to be both secreted, and retained internally, where it may stabilize immature TLR protein expression in the ER, and impact its appearance on the cell surface. Mouse PRAT4B is 245 amino acids (aa) in length. It contains a 27 aa signal sequence plus one coiled-coil region (aa 209 - 237). There is one potential splice variant that shows a 13 aa substitution for the C-terminal nine amino acids. Over aa 30 - 245, mouse PRAT4B is 95% and 87% aa identical to rat and human PRAT4B, respectively.

Long Name

Protein Associated with Toll-like Receptor 4B

Alternate Names

CNPY4

Entrez Gene IDs

245812 (Human); 66455 (Mouse); 363886 (Rat)

Gene Symbol

CNPY4

UniProt

Q8BQ47

Additional PRAT4B Products

Product Documents for Mouse/Rat PRAT4B Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse/Rat PRAT4B Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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