Mouse ROBO4 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse ROBO4 in flow cytometry.
  • Source
    Monoclonal Rat IgG2A Clone # 274914
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Y3 rat myeloid cell line transfected with mouse ROBO4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ROBO4 in Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP by Flow Cytometry. Jurkat human acute T cell leukemia cell line transfected with mouse ROBO4 and eGFP was stained with either (A) Rat Anti-Mouse ROBO4 APC‑conjugated Monoclonal Antibody (Catalog # FAB50041A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROBO4
ROBO4, also known as Magic Roundabout, is a ~150 kDa glycoprotein belonging to the ROBO family. ROBOs are molecular guidance receptors that typically interact with Slit ligands to regulate axon guidance and neuronal migration. Unlike other family members, ROBO4 is mainly restricted to the vascular endothelium. Expression in early hematopoietic progenitors is also reported. Vascular endothelial ROBO4 is expressed at highest levels in during development and vascular remodeling, including tumor angiogenesis. It is proposed to contribute to vascular stability. Consistent with this, endogenous ROBO4 is concentrated in the vascular stalk and sprouts rather than tip cells and appears to protect newly formed blood vessels against VEGF‑induced vascular leak. ROBO4 binding of Slit proteins has been variably reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers. ROBO4 is also variably reported to stimulate or inhibit cell migration or filopodia formation. Effects on cell movement may be mediated through intracellular binding of WASP-, Ras/Rac/Rho-, Mena-, Src-, or Paxillin‑related proteins, all of which affect the cytoskeleton. Recombinant soluble ROBO4 ECD can antagonize endothelial cell migration and in vivo angiogenesis. 
  • Long Name:
    Roundabout Homolog 4
  • Entrez Gene IDs:
    54538 (Human); 74144 (Mouse)
  • Alternate Names:
    ECSM4; FLJ20798; Magic roundabout; MGC133352; MGC133353; MRB; roundabout homolog 4; roundabout homolog 4, magic roundabout (Drosophila)
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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