Mouse ROBO4 APC‑conjugated Antibody

R&D Systems | Catalog # FAB50041A

R&D Systems
Discontinued Product
FAB50041A has been discontinued. View all ROBO4 products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 274914
View all ROBO4 Primary Antibodies »

Product Specifications

Immunogen

Y3 rat myeloid cell line transfected with mouse ROBO4

Specificity

Detects mouse ROBO4 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse ROBO4 APC‑conjugated Antibody

Detection of ROBO4 antibody in Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP antibody by Flow Cytometry.

Detection of ROBO4 in Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP by Flow Cytometry.

Jurkat human acute T cell leukemia cell line transfected with mouse ROBO4 and eGFP was stained with either (A) Rat Anti-Mouse ROBO4 APC-conjugated Monoclonal Antibody (Catalog # FAB50041A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse ROBO4 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Jurkat human acute T cell leukemia cell line transfected with mouse ROBO4 and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROBO4

ROBO4, also known as Magic Roundabout, is a ~150 kDa glycoprotein belonging to the ROBO family. ROBOs are molecular guidance receptors that typically interact with Slit ligands to regulate axon guidance and neuronal migration. Unlike other family members, ROBO4 is mainly restricted to the vascular endothelium. Expression in early hematopoietic progenitors is also reported. Vascular endothelial ROBO4 is expressed at highest levels in during development and vascular remodeling, including tumor angiogenesis. It is proposed to contribute to vascular stability. Consistent with this, endogenous ROBO4 is concentrated in the vascular stalk and sprouts rather than tip cells and appears to protect newly formed blood vessels against VEGF‑induced vascular leak. ROBO4 binding of Slit proteins has been variably reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers. ROBO4 is also variably reported to stimulate or inhibit cell migration or filopodia formation. Effects on cell movement may be mediated through intracellular binding of WASP-, Ras/Rac/Rho-, Mena-, Src-, or Paxillin‑related proteins, all of which affect the cytoskeleton. Recombinant soluble ROBO4 ECD can antagonize endothelial cell migration and in vivo angiogenesis. 

Long Name

Roundabout Homolog 4

Alternate Names

ECSM4, FLJ20798, Magic roundabout, MGC133352, MGC133353, MRB, roundabout homolog 4, roundabout homolog 4, magic roundabout (Drosophila)

Entrez Gene IDs

54538 (Human); 74144 (Mouse)

Gene Symbol

ROBO4

Additional ROBO4 Products

Product Documents for Mouse ROBO4 APC‑conjugated Antibody

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Product Specific Notices for Mouse ROBO4 APC‑conjugated Antibody

For research use only

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