Detection of ROBO4 in Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP by Flow Cytometry.
Jurkat human acute T cell leukemia cell line transfected with mouse ROBO4 and eGFP was stained with either (A) Rat Anti-Mouse ROBO4 APC‑conjugated Monoclonal Antibody (Catalog # FAB50041A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
also known as Magic Roundabout, is a ~150 kDa glycoprotein belonging to
the ROBO family. ROBOs are molecular guidance receptors that
typically interact with Slit ligands to regulate axon guidance and
neuronal migration. Unlike other family members, ROBO4 is mainly
restricted to the vascular endothelium. Expression in early
hematopoietic progenitors is also reported. Vascular endothelial ROBO4 is expressed at
highest levels in during development and vascular remodeling, including
tumor angiogenesis. It is proposed to contribute to
vascular stability. Consistent with this, endogenous ROBO4 is
concentrated in the vascular stalk and sprouts rather than tip cells and
appears to protect newly formed blood vessels against VEGF‑induced
vascular leak. ROBO4 binding of Slit proteins has been variably
reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers. ROBO4 is also variably reported to stimulate or
inhibit cell migration or filopodia formation. Effects on
cell movement may be mediated through intracellular binding of WASP-,
Ras/Rac/Rho-, Mena-, Src-, or Paxillin‑related proteins, all of which
affect the cytoskeleton. Recombinant soluble ROBO4 ECD
can antagonize endothelial cell migration and in vivo
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