Mouse Semaphorin 4F APC-conjugated Antibody

Name: Mouse Semaphorin 4F APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB7200A
Price: 469 USD

Catalog #: FAB7200A Datasheet / COA / SDS

Newer Version Available: FAB7200R

NEW

Discontinued Product

FAB7200A has been discontinued and is replaced by FAB7200R.

Detection of Semaphorin 4F in Mouse Cortical Stem Cells and bEnd.3 Mouse Cell Line by Flow Cytometry.

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Mouse Semaphorin 4F APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Semaphorin 4 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 780225

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4F
Arg41-Gly667
Accession # Q9Z123

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Semaphorin 4F in Mouse Cortical Stem Cells and bEnd.3 Mouse Cell Line by Flow Cytometry. Mouse cortical stem cells (A) and bEnd.3 mouse endothelioma cell line (B) were stained with Rat Anti-Mouse Semaphorin 4F APC-conjugated Monoclonal Antibody (Catalog # FAB7200A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Semaphorin 4F

Semaphorin 4F (Sema4F), also known as Sema W, is a 90-100 kDa class IV member of the semaphorin family of proteins. It is expressed by select cell types, including Schwann cells and neurons, and likely acts as an inhibitor of cell proliferation by Schwann cells, and a regulator of postsynaptic glutamatergic synapses on neuron dendrites. Mature mouse Sema4F is a type I transmembrane protein that is 737 amino acids (aa) in length. It contains a 627 aa extracellular region (aa 41-667) that is characterized by the presence of one Sema domain (aa 71-493), a PSI region (aa 518-569), and a C2-type Ig-like domain (aa 586-641). Over aa 41-667, mouse Sema4F shares 92% and 98% aa sequence identity with human and rat Sema4F, respectively.

Entrez Gene IDs

10505 (Human); 20355 (Mouse); 29745 (Rat)

Alternate Names

M-SEMA; m-Sema-M; PRO2353; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4F; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4F; Sema M; Sema W; SEMA4F; SEMAM; Semaphorin 4F; semaphorin-4F; semaphorin-M; semaphorin-W; SEMAW; SEMAWsemaphorin M

Product Datasheets

Product Datasheet

COA

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Related Research Areas

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