Detection of Semaphorin 4F in Mouse Cortical Stem Cells and bEnd.3 Mouse Cell Line by Flow Cytometry.
Mouse cortical stem cells (A) and bEnd.3 mouse endothelioma cell line (B) were stained with Rat Anti-Mouse Semaphorin 4F APC‑conjugated Monoclonal Antibody (Catalog # FAB7200A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 4F
Semaphorin 4F (Sema4F), also known as Sema W, is a 90-100 kDa class IV member of the semaphorin family of proteins. It is expressed by select cell types, including Schwann cells and neurons, and likely acts as an inhibitor of cell proliferation by Schwann cells, and a regulator of postsynaptic glutamatergic synapses on neuron dendrites. Mature mouse Sema4F is a type I transmembrane protein that is 737 amino acids (aa) in length. It contains a 627 aa extracellular region (aa 41-667) that is characterized by the presence of one Sema domain (aa 71-493), a PSI region (aa 518-569), and a C2-type Ig-like domain (aa 586-641). Over aa 41-667, mouse Sema4F shares 92% and 98% aa sequence identity with human and rat Sema4F, respectively.
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