Mouse Semaphorin 4F APC-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Semaphorin 4 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 780225
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4F 
    Arg41-Gly667
    Accession # Q9Z123
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Semaphorin 4F in Mouse Cortical Stem Cells and bEnd.3 Mouse Cell Line by Flow Cytometry. Mouse cortical stem cells (A) and bEnd.3 mouse endothelioma cell line (B) were stained with Rat Anti-Mouse Semaphorin 4F APC‑conjugated Monoclonal Antibody (Catalog # FAB7200A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 4F

Semaphorin 4F (Sema4F), also known as Sema W, is a 90-100 kDa class IV member of the semaphorin family of proteins. It is expressed by select cell types, including Schwann cells and neurons, and likely acts as an inhibitor of cell proliferation by Schwann cells, and a regulator of postsynaptic glutamatergic synapses on neuron dendrites. Mature mouse Sema4F is a type I transmembrane protein that is 737 amino acids (aa) in length. It contains a 627 aa extracellular region (aa 41-667) that is characterized by the presence of one Sema domain (aa 71-493), a PSI region (aa 518-569), and a C2-type Ig-like domain (aa 586-641). Over aa 41-667, mouse Sema4F shares 92% and 98% aa sequence identity with human and rat Sema4F, respectively.

               

  • Entrez Gene IDs:
    10505 (Human); 20355 (Mouse); 29745 (Rat)
  • Alternate Names:
    M-SEMA; m-Sema-M; PRO2353; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4F; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4F; Sema M; Sema W; SEMA4F; SEMAM; Semaphorin 4F; semaphorin-4F; semaphorin-M; semaphorin-W; SEMAW; SEMAWsemaphorin M
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 7  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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