Mouse sL-Selectin/CD62L Quantikine ELISA Kit

Name: Mouse sL-Selectin/CD62L Quantikine ELISA Kit
Brand: R&D Systems
SKU: MLS00

R&D Systems | Catalog # MLS00

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Product Documents

Discontinued Product

MLS00 has been discontinued. View all L-Selectin/CD62L products.

Product Summary
Product Specifications
Precision
Recovery
Linearity
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)

Sensitivity

0.034 ng/mL

Assay Range

0.62-40 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma)

View all L-Selectin/CD62L ELISA Kits »

Product Summary for Mouse sL-Selectin/CD62L Quantikine ELISA Kit

The Quantikine Mouse soluble L-Selectin (sL-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sL-Selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse L-Selectin and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sL-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sL-Selectin.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Species

Mouse

Specificity

Natural and recombinant mouse sL-Selectin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.43	6.95	18.2	1.63	7.43	19.3
Standard Deviation	0.07	0.50	1.5	0.10	0.50	1.5
CV%	4.9	7.2	8.2	6.1	6.7	7.8

Recovery for Mouse sL-Selectin/CD62L Quantikine ELISA Kit

The recovery of mouse sL-Selectin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	94	83-109
EDTA Plasma (n=6)	93	80-119
Serum (n=10)	93	80-116

Linearity

To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse sL-Selectin in each matrix were diluted with Calibrator Diluent and then assayed.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: L-Selectin/CD62L

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.