Home / L-Selectin/CD62L / Mouse sL-Selectin/CD62L Quantikine ELISA Kit

Mouse sL-Selectin/CD62L Quantikine ELISA Kit

Catalog #: MLS00
8 Citations Datasheet / COA / SDS

Discontinued Product

MLS00 has been discontinued.
View all L-Selectin/CD62L products.
1 Image
Product Details
Procedure
Citations (8)
FAQs
Reviews
Summary Precision Recovery Linearity Product Datasheets Preparation and Storage Background Related Research Areas

Mouse sL-Selectin/CD62L Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)
Sensitivity
0.034 ng/mL
Assay Range
0.6 - 40 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse sL-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse soluble L-Selectin (sL-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sL-Selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse L-Selectin and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sL-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sL-Selectin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.43 6.95 18.2 1.63 7.43 19.3
Standard Deviation 0.07 0.5 1.5 0.1 0.5 1.5
CV% 4.9 7.2 8.2 6.1 6.7 7.8

Recovery

The recovery of mouse sL-Selectin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 94 83-109
EDTA Plasma (n=6) 93 80-119
Serum (n=10) 93 80-116

Linearity

To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse sL-Selectin in each matrix were diluted with Calibrator Diluent and then assayed.

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: L-Selectin/CD62L

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.

Entrez Gene IDs:
6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)
Alternate Names:
CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse sL-Selectin/CD62L Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

  1. Inhibition of myeloperoxidase decreases vascular oxidative stress and increases vasodilatation in sickle cell disease mice.
    Authors: Zhang H, Xu H, Weihrauch D, Jones D, Jing X, Shi Y, Gourlay D, Oldham K, Hillery C, Pritchard K
    J Lipid Res, 2013-08-16;54(11):3009-15.
    Species: Mouse
    Sample Types: Plasma
  2. In vivo modulation of the inflammatory response by nonsteroidal antiinflammatory drug-related compounds that trigger L-selectin shedding.
    Authors: Herrera-Garcia A, Dominguez-Luis M, Arce-Franco M, Lopez-Fernandez J, Feria M, Barreiro O, Sanchez-Madrid F, Diaz-Gonzalez F
    Eur J Immunol, 2012-10-26;43(1):55-64.
    Species: Mouse
    Sample Types: Plasma
  3. Attenuation of transplant arteriosclerosis with clopidogrel is associated with a reduction of infiltrating dendritic cells and macrophages in murine aortic allografts.
    Authors: Abele S, Spriewald BM, Ramsperger-Gleixner M, Wollin M, Hiemann NE, Nieswandt B, Weyand M, Ensminger SM
    Transplantation, 2009-01-27;87(2):207-16.
    Species: Mouse
    Sample Types: Serum
  4. Inflamed phenotype of the mesenteric microcirculation of melanocortin type 3 receptor-null mice after ischemia-reperfusion.
    Authors: Leoni G, Patel HB, Sampaio AL, Gavins FN, Murray JF, Grieco P, Getting SJ, Perretti M
    FASEB J., 2008-08-29;22(12):4228-38.
    Species: Mouse
    Sample Types: Plasma
  5. Myeloid Src kinases regulate phagocytosis and oxidative burst in pneumococcal meningitis by activating NADPH oxidase.
    Authors: Paul R, Obermaier B, Van Ziffle J, Angele B, Pfister HW, Lowell CA, Koedel U
    J. Leukoc. Biol., 2008-07-14;84(4):1141-50.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. Selenium supplementation induces metalloproteinase-dependent L-selectin shedding from monocytes.
    Authors: Ahrens I, Ellwanger C, Smith BK, Bassler N, Chen YC, Neudorfer I, Ludwig A, Bode C, Peter K
    J. Leukoc. Biol., 2008-02-27;83(6):1388-95.
    Species: Mouse
    Sample Types: Serum
  7. Atheroprotective role of interleukin-6 in diet- and/or pathogen-associated atherosclerosis using an ApoE heterozygote murine model.
    Authors: Madan M, Bishayi B, Hoge M, Amar S
    Atherosclerosis, 2007-04-06;197(2):504-14.
    Species: Mouse
    Sample Types: Serum
  8. ADAM17 deficiency by mature neutrophils has differential effects on L-selectin shedding.
    Authors: Li Y, Brazzell J, Herrera A, Walcheck B
    Blood, 2006-05-30;108(7):2275-9.
    Species: Mouse
    Sample Types: Plasma

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Reviews for Mouse sL-Selectin/CD62L Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Mouse sL-Selectin/CD62L Quantikine ELISA Kit and earn rewards!

Have you used Mouse sL-Selectin/CD62L Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review