Detects mouse Themis in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) Themis (aa 2‑282) is observed under non-reducing conditions, and approximately 10% cross-reactivity with rhThemis (aa 2‑282) is observed under reducing conditions.
Monoclonal Rat IgG2B Clone # 719945
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Themis Lys122-Lys237 Accession # Q8BGW0
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488
Intracellular Staining by Flow Cytometry
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Themis in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse Themis Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6816G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Themis (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid (aa) cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling. Within the region used as an immunogen, mouse Themis shares 87% and 96% aa sequence identity with human and rat Themis, respectively.
Thymocyte-expressed Molecule Involved in Selection
Entrez Gene IDs:
387357 (Human); 210757 (Mouse)
bA325O24.3; bA325O24.4; C6orf190; C6orf207; FLJ40584; Gasp; GRB2-associated protein; MGC163388; protein THEMIS; signaling phosphoprotein specific for T cells; SPOT; thymocyte selection associated; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; TSEPA
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