Mouse TWEAK R/TNFRSF12 Alexa Fluor® 405-conjugated Antibody

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FAB16101V-100UG
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Mouse TWEAK R/TNFRSF12 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TWEAK R/TNFRSF12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) EDA, rmNeogenin, recombinant human TWEAK R, rmOPG, or rmTNF R1 is observed.
Source
Monoclonal Rat IgG2a Clone # 314636
Immunogen
E. coli-derived recombinant mouse TWEAK R
Glu28-Trp79
Accession # Q9CR75.1
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
MS‑1 mouse pancreatic islet endothelial cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TWEAK R/TNFRSF12

TNF-related weak inducer of apoptosis receptor (TWEAK R) belongs to the TNF receptor superfamily and is designated TNFRSF12. The gene for TWEAK R was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Mouse TWEAK R cDNA encodes a 129 amino acid (aa) residues type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1‑3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain contains one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death by both caspase-dependent apoptosis and cathepsin B-dependent necrosis, cell proliferation, and angiogenesis (2‑6). In newborn mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

References
  1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
  2. Feng, S. et al. (2000) Am. J. Pathol. 156:1253.
  3. Wiley, S. et al. (2001) Immunity 15:837.
  4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
  5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
  6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.
Long Name
TNF-like and Weak Inducer of Apoptosis Receptor
Entrez Gene IDs
51330 (Human); 27279 (Mouse)
Alternate Names
CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14

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