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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Between 1087 and 1137
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MSH3 Antibody - BSA Free
Immunoprecipitation:MSH3 AntibodyNBP3-28987] -
Immunoprecipitation:MSH3 AntibodyNBP3-28987] -Detection of human MSH3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MSH3 antibody NBP3-28987 (lot NBP3-28987-1) used for IP at 6 µg per reaction. MSH3 was also immunoprecipitated by rabbit anti-MSH3 antibody. For blotting immunoprecipitated MSH3, NBP3-28987 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.MSH3 AntibodyNBP3-28987] -
Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkatcells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-MSH3 antibody A305-314A used for WB at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.Applications for MSH3 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 µg/mg lysate
Western Blot
1:2,000 - 1:10,000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate pH 7 -8.
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MSH3
Alternate Names
Divergent upstream protein, DNA mismatch repair protein Msh3, DUC1, DUG, DUPMGC163306, hMSH3, Mismatch repair protein 1, MRP1MGC163308, mutS (E. coli) homolog 3, mutS homolog 3 (E. coli)
Gene Symbol
MSH3
Additional MSH3 Products
Product Documents for MSH3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MSH3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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