MSH6 Antibody (035) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-89265
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunocytochemistry-Immunofluorescence-NBP2-89265-img0003.jpg "Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (035) [NBP2-89265]")
Key Product Details
Species Reactivity
Human, Mouse, Rat, Ferret, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 035 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human MSH6.
Reactivity Notes
Cross reactivity in IHC:
Cynomolgus, Mouse, Rat, Ferret
Cynomolgus, Mouse, Rat, Ferret
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for MSH6 Antibody (035) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (035) [NBP2-89265]
Immunocytochemistry/Immunofluorescence: MSH6 Antibody (035) [NBP2-89265] - Staining of MSH6 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MSH6 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.![Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunohistochemistry-Paraffin-NBP2-89265-img0011.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]")
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in Ferret kidney with rabbit monoclonal antibody at 1:200 dilution.![Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunohistochemistry-Paraffin-NBP2-89265-img0001.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]")
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in human rectal cancer with rabbit monoclonal antibody (1:200).![Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunohistochemistry-Paraffin-NBP2-89265-img0002.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]")
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in human colon carcinoma with rabbit monoclonal antibody (1:200).![Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunohistochemistry-Paraffin-NBP2-89265-img0004.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]")
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in cynomolgus spleen with rabbit monoclonal antibody at 1:200 dilution.![Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunohistochemistry-Paraffin-NBP2-89265-img0005.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]")
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in cynomolgus kidney with rabbit monoclonal antibody at 1:200 dilution.![Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunohistochemistry-Paraffin-NBP2-89265-img0006.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]")
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in mouse spleen with rabbit monoclonal antibody at 1:200 dilution.![Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunohistochemistry-Paraffin-NBP2-89265-img0007.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]")
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in mouse kidney with rabbit monoclonal antibody at 1:200 dilution.![Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunohistochemistry-Paraffin-NBP2-89265-img0008.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]")
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in rat spleen with rabbit monoclonal antibody at 1:200 dilution.![Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunohistochemistry-Paraffin-NBP2-89265-img0009.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]")
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in rat kidney with rabbit monoclonal antibody at 1:200 dilution![Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]](https://resources.rndsystems.com/images/products/MSH6-Antibody-035-Immunohistochemistry-Paraffin-NBP2-89265-img0010.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]")
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in Ferret spleen with rabbit monoclonal antibody at 1:200 dilution.Applications for MSH6 Antibody (035) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MSH6
Alternate Names
DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein
Gene Symbol
MSH6
Additional MSH6 Products
Product Documents for MSH6 Antibody (035) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MSH6 Antibody (035) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for MSH6 Antibody (035) - Azide and BSA Free
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Q: I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.
A: Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.
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