MTT

Catalog # Availability Size / Price Qty
5224/500
Buffers, Chelators and Reagents Small Molecule
1 Image
Description: Reagent used in the measurement of cell proliferation

Chemical Name: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide

Purity: ≥98%

Product Details
Reviews (2)

Biological Activity

Reagent used in the measurement of cell proliferation.

Technical Data

M.Wt:
414.32
Formula:
C18H16N5SBr
Solubility:
Soluble to 5 mg/ml in water
Purity:
≥98%
Storage:
Store at +4°C
CAS No:
298-93-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Detection of apoptosis via cell proliferation assay MTT was carried out
By Anonymous on 02/11/2019
Species: Human

Detection of apoptosis via cell proliferation assay MTT was carried out.The 10 µl solution of MTT is added to one of the 96 well- plate and then incubate the plate for 4 hours. After incubation time the medium from wells was discarded and 100 µl of acid-propanol added to all well of the plate and incubated for 30 minutes at room temperature. Then the absorbance of each well were measured at 340 nm by plate reader.


MTT as a tool to estimate cell proliferation
By Anonymous on 12/12/2018
Species: Mouse

HL-1 cells were treated with 15d-PGJ2 (15 μM) for 0-12 h followed by incubation with MTT (0.5 mg/ml; dissolved in serum-free medium) for 30 min at 37°C. The converted insoluble dark purple coloured dye (formazan) was solubilized with 300 µl acidic isopropanol (0.04 M HCl in absolute isopropanol). Cell proliferation was assessed by measuring optical densities at emission and correction wavelengths of 570 and 630 nm, respectively, on a microtiter plate reader.


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