R&D Systems' new MycoProbe® Mycoplasma Detection Kit (Catalog # CUL001B) represents a major breakthrough for high-throughput, routine screening of cell cultures for mycoplasma contamination. Mycoplasma contamination is one of the most common and serious problems in culturing eukaryotic cells because it alters the phenotypic characteristics of the cells and can negatively impact results. Since mycoplasma is typically not visible and does not respond to antibiotics, an alternative sensitive and reliable detection method is required. This assay is capable of detecting the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination.
|Table 1. Comparison of R&D Systems MycoProbe® Mycoplasma Detection Assay (Catalog # CUL001B), with a commercially available PCR-based mycoplasma detection kit. Serial dilutions of M.arginini were produced in lysis buffer.
*CFU = Colony Forming Units per well or PCR reaction