Recombinant Human Myosin Light Chain 2 His Protein

Name: Recombinant Human Myosin Light Chain 2 His Protein
Brand: Novus Biologicals
SKU: NBC1-18536
Price: 349 USD
Availability: InStock

Novus Biologicals | Catalog # NBC1-18536

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all Myosin Light Chain 2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids1-166 of Human Myosin Light Chain 2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPKKAKKRA GGANSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGVLKADYVR EMLTTQAERF SKEEVDQMFA AFPPDVTGNL DYKNLVHIIT HGEEKD

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Myosin Light Chain 2 His Protein

SDS-PAGE: Recombinant Human Myosin Light Chain 2 His Protein [NBC1-18536]

SDS-Page: Recombinant Human Myosin Light Chain 2 Protein [NBC1-18536] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 20.9 kDa (186aa), confirmed by MALDI-TOF.Concentration: 0.5 mg/ml (determined by Bradford assay).

Formulation, Preparation, and Storage

NBC1-18536

Formulation	20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 5mM CaCl2
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin Light Chain 2

Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. Recombinant human MYL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

cardiac ventricular myosin light chain 2, CMH10myosin light chain 2, DKFZp779C0562, MLC2, MLC-2, MLC-2v, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosin, light chain 2, regulatory, cardiac, slow, myosin, light polypeptide 2, regulatory, cardiac, slow, regulatory light chain of myosin, RLC of myosin, slow cardiac myosin regulatory light chain 2

Gene Symbol

MYL2

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Product Documents for Recombinant Human Myosin Light Chain 2 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Myosin Light Chain 2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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