Myosin Phosphatase 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87751

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Myosin Phosphatase 2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GTGINFWTKDEDETDGSEEVKETWHERLSRLESGGSNPTTSDSYGDRASARARREAREARLATLTSRVEEDSNRDYKKLYESALTENQKLKTKLQEAQLELADIKS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin Phosphatase 2 Antibody - BSA Free

Western Blot: Myosin Phosphatase 2 Antibody [NBP1-87751]

Western Blot: Myosin Phosphatase 2 Antibody [NBP1-87751]

Western Blot: Myosin Phosphatase 2 Antibody [NBP1-87751] - Analysis in control (vector only transfected HEK293T lysate) and PPP1R12B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751]

Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751]

Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751] - Staining in human heart muscle and pancreas tissues using anti-PPP1R12B antibody. Corresponding PPP1R12B RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751]

Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751]

Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751]

Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751]

Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751] - Staining of human pancreas shows low expression as expected.

Applications for Myosin Phosphatase 2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin Phosphatase 2

PPP1R12B, also known as Protein phosphatase 1 regulatory subunit 12B, has 5 isoforms, a 982 amino acid isoform that is 110 kDa, a 386 amino acid isoform that is 43 kDa, a 224 amino acid isoform that is 26 kDa, a 208 amino acid isoform that is 24 kDa, and a 515 amino acid isoform that is 58 kDa; detected in skeletal muscle, fetal and adult heart, brain, placenta, kidney, spleen, thymus, pancreas and lung; with cytoplasmatic subcellular location; regulates myosin phosphatase activity and augments Ca(2+) sensitivity of the contractile apparatus. This protein is being studied for its involvement in breast cancer. The PPP1R12B protein has been shown to involve IL16, PPP1CB, PRKG1, HSPA8, MYL6, and MYL6B in RhoA pathway, PKA signaling, actin-based motility by Rho family GTPases, eIF2 pathway, CDK5 pathway, insulin pathway, G2/M transition, cell cycle, mitotic G2-G2/M phases, regulation of PLK1 activity at G2/M transition, cell cycle, mitotic, and vascular smooth muscle contraction pathways.

Alternate Names

MGC131980, MGC87886, Myosin phosphatase target subunit 2, myosin phosphatase, target subunit 2, Myosin phosphatase-targeting subunit 2, MYPT 2, MYPT2FLJ40942, protein phosphatase 1 regulatory subunit 12B, protein phosphatase 1, regulatory (inhibitor) subunit 12B

Gene Symbol

PPP1R12B

Additional Myosin Phosphatase 2 Products

Product Documents for Myosin Phosphatase 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Myosin Phosphatase 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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