NARS Antibody - BSA Free

Western Blot: NARS Antibody - BSA Free [NBP1-31896] -

Patients cells display loss of NARS1 functions.a Fibroblast cells from two unaffected (U1 and U2) and two affected (A1 and A2) show reduced levels compared with actin control. b Quantification results for WB, n = 3, Error bar: +/- SD. c Overexpression in cDNA expressing patient variants in HEK293T cells showed unstable NARS1 protein. d Reduced NARS1 tRNA synthetase activity in patient fibroblasts. n = 3, Error bar: +/- SD. e Other tRNA-ARS activity not impaired in NARS1 mutated cells. TARS: Threonyl-tRNA synthetase; KARS: lysyl-tRNA synthetase; RARS: arginyl-tRNA synthetase. n = 3, Error bar: +/- SD. f Patient IPSCs showed reduced puromycin incorporation after 1 h pulse, indicating reduced protein synthesis. For the quantification data in (b) and (d), n = 3 represents three independent biological replicates, two-tailed p values based upon Student t-test with Holm–Šidak multiple comparison correction, and individual p value was shown in (b, d); Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32788587), licensed under a CC-BY license. Not internally tested by Novus Biologicals.