NBD-PE

Tocris Bioscience | Catalog # 7538

Green-fluorescent label for lysosomal lipid bodies. Used for: Application: confocal microscopy
Tocris Bioscience

Key Product Details

Description

Green-fluorescent label for lysosomal lipid bodies. Used for: Application: confocal microscopy

Wavelength

Excitation = 463 nm, Emission = 536 nm
View all Fluorescent Lipids Probes and Cell Membrane Stains »

Product Description

Key information: NBD-PE is a fluorescent label for lysosomal lipid bodies.

Used for: quantitatively measure phospholipidosis, which is useful to screen drugs for SARS-CoV-2 repurposing. NBD-PE can also be used to label giant unilamellar vesicles (GUV).

Application: confocal microscopy, fluorescence recovery after photobleaching (FRAP).

Properties and Photophysical Data: It consists of a single tail (16:0) phospholipid derivative with an NBD fluorophore conjugated to the head group. Excitation and emission maxima (λ) are 463 nm and 536 nm, respectively, extinction coefficient = 22,000 M-1cm-1.

Optical Data

Emission Color Green
λabs 463 nm
λem 536 nm
Extinction Coefficient (ε) 22000 M-1cm-1
Application Confocal Microscopy

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for NBD-PE

Molecular Weight

956.26

Formula

C43H75N4O11P.C6H15N

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

Triethylammonium (R)-2,3-bis(palmitoyloxy)propyl(2-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)ethyl)phosphate

CAS Number

178119-00-1

InChI Key

LEJHDNAGUHKMLR-GKEJWYBXSA-N

SMILES

CC[N+](CC)([H])CC.CCCCCCCCCCCCCCCC(OC[C@@H](OC(CCCCCCCCCCCCCCC)=O)COP([O-])(OCCNC1=CC=C(N(=O)=O)C2=NON=C12)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
methanol 0.48 0.5 with sonication

Preparing Stock Solutions for NBD-PE

The following data is based on the product molecular weight 956.26.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.005 mM 209.15 mL 1,045.74 mL 2,091.48 mL
0.025 mM 41.83 mL 209.15 mL 418.30 mL
0.05 mM 20.91 mL 104.57 mL 209.15 mL
0.25 mM 4.18 mL 20.91 mL 41.83 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product.

Product Documents for NBD-PE

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NBD-PE

For research use only

Customer Reviews for NBD-PE

There are currently no reviews for this product. Be the first to review NBD-PE and earn rewards!

Have you used NBD-PE?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card