Necrostatin-1

Tocris Bioscience | Catalog # 2324

RIP1 kinase inhibitor; inhibits necroptosis
Tocris Bioscience

Key Product Details

Description

RIP1 kinase inhibitor; inhibits necroptosis
View all RIP Kinase Inhibitors »

Product Description

Necrostatin-1 is an ATP-competitive, allosteric inhibitor of receptor-interacting protein kinase 1 (RIPK1) (EC50 = 182 nM). Blocks non-apoptotic cell death via inhibition of a specific cellular pathway, necroptosis, which leads to necrosis (EC50 = 494 nM). Reduces ischemic brain injury in a mouse model of stroke.

Product Specifications for Necrostatin-1

Molecular Weight

259.33

Formula

C13H13N3OS

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone

CAS Number

4311-88-0

PubChem ID

2828334

InChI Key

TXUWMXQFNYDOEZ-UHFFFAOYSA-N

SMILES

CN1C(=S)NC(CC2=CNC3=CC=CC=C23)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.78 30

Preparing Stock Solutions for Necrostatin-1

The following data is based on the product molecular weight 259.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.3 mM 12.85 mL 64.27 mL 128.54 mL
1.5 mM 2.57 mL 12.85 mL 25.71 mL
3 mM 1.29 mL 6.43 mL 12.85 mL
15 mM 0.26 mL 1.29 mL 2.57 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 56 publications citing the usage of this product.

Product Documents for Necrostatin-1

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Necrostatin-1

For research use only

Customer Reviews for Necrostatin-1 (2)

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  • Necroptosis-related studies
    Name: MARINA KOUTSIOUMPA
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 10/13/2022
    Combinatorial treatments of Necrostatin-1 with other chemotherapeutic drugs were performed to elucidate the dependance of the latter on the induction of necroptosis. Results were consistent.
  • good necroptosis inhibitor
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Caco-2
    Verified Customer | Posted 06/28/2020
    100nM of Nec-1 was used to inhibit RIPK1 after administration cells with TNFa, zVADfmk, and smac inhibitors to induce necroptosis, then cell death rate was measured with a commercial kit.
    Necrostatin-1 2324

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