Nectin-4/PVRL4 Antibody (DMC438) - Chimeric

Novus Biologicals | Catalog # NBP3-48336

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC438
View all Nectin-4 Primary Antibodies »

Product Specifications

Immunogen

Nectin-4/PVRL4 recombinant protein expressed in mammalian cells (Accession# Q96NY8). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for Nectin-4/PVRL4 Antibody (DMC438) - Chimeric

Nectin-4/PVRL4 Antibody (DMC438) - Chimeric

Flow Cytometry: Nectin-4/PVRL4 Antibody (DMC438) - Chimeric [NBP3-48336]

Flow cytometry analysis of antigen binding of anti-human Nectin4 mAb(NBP3-48336). (A) NBP3-48336 does not bind to 293T cells that do not express Nectin4. (B) A clear peak shift of NBP3-48336 was seen compared to the control when incubated with Nectin4-expressing A431 cells, indicating strong binding of NBP3-48336 to Nectin4. Antibodies were incubated at 2 ug/mL.
Nectin-4/PVRL4 Antibody (DMC438) - Chimeric

Flow Cytometry: Nectin-4/PVRL4 Antibody (DMC438) - Chimeric [NBP3-48336]

Flow cytometry analysis with Anti-Nectin-4/PVRL4 (DMC438) on HEK293 cells transfected with human Nectin-4/PVRL4 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Applications for Nectin-4/PVRL4 Antibody (DMC438) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nectin-4

PVRL4, also known as Poliovirus receptor-related protein 4, is a 510 amino acid protein that is 55 kDa, predominantly expressed in placenta and breast carcinoma, is a single-pass type I membrane protein involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1, and oes not act as receptor for alpha-herpesvirus entry into cells. Disease research is being performed in relation to this protein and ectodermal dysplasia, syndactyly, ectodermal dysplasia-syndactyly syndrome 1, measles, ovarian cancer, breast carcinoma, lung cancer, and carcinoma. The PVRL4 protein has shown an interaction with PVRL1, MLLT4, PICK1, TIGIT, PARD3, PVRL3, and PVRL2 in Adherens junctions interactions, Nectin/Necl trans heterodimerization, Cell junction organization, Cell-Cell communication, Cell-cell junction organization, Sertoli-Sertoli Cell Junction Dynamics, and Epithelial Adherens Junctions pathways.

Long Name

Poliovirus Receptor Related 4

Alternate Names

LNIR, Nectin4, PRR4, PVRL4

Gene Symbol

NECTIN4

Additional Nectin-4 Products

Product Documents for Nectin-4/PVRL4 Antibody (DMC438) - Chimeric

Certificate of Analysis

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Product Specific Notices for Nectin-4/PVRL4 Antibody (DMC438) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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