NEK6 Antibody (1V1X8)

Novus Biologicals | Catalog # NBP3-16215

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1V1X8 expressed in HEK293
View all NEK6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human NEK6 (Q9HC98). ETTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLFSLCQKIEQCDYPPLPGEHYSEKLRELVSMCICPDPHQRPDIGYVH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEK6 Antibody (1V1X8)

Western Blot: NEK6 Antibody (1V1X8) [NBP3-16215]

Western Blot: NEK6 Antibody (1V1X8) [NBP3-16215]

Western Blot: NEK6 Antibody (1V1X8) [NBP3-16215] - Western blot analysis of extracts of various cell lines, using NEK6 Rabbit mAb (NBP3-16215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
Immunohistochemistry-Paraffin: NEK6 Antibody (1V1X8) [NBP3-16215]

Immunohistochemistry-Paraffin: NEK6 Antibody (1V1X8) [NBP3-16215]

Immunohistochemistry-Paraffin: NEK6 Antibody (1V1X8) [NBP3-16215] - Immunohistochemistry of paraffin-embedded rat kidney using NEK6 Rabbit mAb (NBP3-16215) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: NEK6 Antibody (1V1X8) [NBP3-16215]

Western Blot: NEK6 Antibody (1V1X8) [NBP3-16215]

Western Blot: NEK6 Antibody (1V1X8) [NBP3-16215] - Western blot analysis of extracts of various cell lines, using NEK6 Rabbit mAb (NBP3-16215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for NEK6 Antibody (1V1X8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEK6

NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain. The protein is believed to regulate chromatin condensation in mammalian cells via phosphorylation of histones H1 and H3, and it has been shown to phosphorylate ribosomal S6 protein kinase (S6K1) and serum and glucocorticoid induced protein kinase (SGK1), which regulate diverse cellular processes. The protein also has been shown to bind NEK9, another NIMA-related kinase which regulates chromosome alignment and segregation. At least three mRNA transcripts have been identified, 1.6-, 2.6-, and 9.5-kb. The NEK6 gene is localized to 9q33-34, a region at which the loss of heterozygosity is associated with transitional cell carcinomas. NEK6 expression has been documented in many normal human tissues, including blood, brain, heart, liver, lung, skeletal muscle, spleen and testis. ESTs have been isolated from numerous tissue libraries, especially normal cervix and amnion and cancerous bone marrow.

Alternate Names

EC 2.7.11.1, Never in mitosis A-related kinase 6, NIMA (never in mitosis gene a)-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512, putative serine-threonine protein kinase, serine/threonine-protein kinase Nek6, SID6-1512

Gene Symbol

NEK6

Additional NEK6 Products

Product Documents for NEK6 Antibody (1V1X8)

Certificate of Analysis

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Product Specific Notices for NEK6 Antibody (1V1X8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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