NeuroTACS In Situ Apoptosis Detection Kit

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4823-30-K
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NeuroTACS In Situ Apoptosis Detection Kit Summary

Provides rapid and convenient identification of apoptosis in brain tissue or neuronal cells using a TUNEL based assay.

Key Benefits

• Unique buffer system produces more consistent labeling.
• Performance tested on brain sections.
• Includes exclusive NeuroPore permeabilization reagent.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
• Helps resolve unique problems encountered when detecting apoptotic neuronal cells.

Why Use the NeuroTACS In Situ Apoptosis Detection Kit?

The kit has been developed to overcome the common difficulties unique to neuronal samples including the fragile nature of brain tissue sections, high background problems, poor counterstaining with common dyes, and the need to perform dual labeling experiments to detect cell specific antigens in conjunction with apoptotic cells. A key feature is NeuroPore, a proprietary permeabilization reagent that gently permeabilizes samples while retaining cell morphology. NeuroPore also contains blocking reagents to allow its use as an antibody diluent in immunohistochemistry and to reduce background staining.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• Diaminobenzidine
• DAB Enhancer
• TACS-Nuclease
• TACS-Nuclease Buffer
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Manganese Cation
• NeuroPore
• Blue Counterstain

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

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