NFkB p105/p50 Antibody (RM299)

Name: NFkB p105/p50 Antibody (RM299)
Brand: Novus Biologicals
SKU: NBP2-61568
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-61568

Submit a review

Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM299 expressed in HEK293

View all NFkB p105/p50 Primary Antibodies »

Product Specifications

Immunogen

A peptide corresponding to residues in Human NFkB p105 subunit and p50 subunit.

Reactivity Notes

This antibody may also react to mouse or rat NFkB p105/p50, as predicted by immunogen homology.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFkB p105/p50 Antibody (RM299)

Western Blot: NFkB p105/p50 Antibody (RM299) [NBP2-61568]

Western Blot: NFkB p105/p50 Antibody (RM299) [NBP2-61568] - HeLa cell lysate using NBP2-61568.

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (RM299) [NBP2-61568]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (RM299) [NBP2-61568] - Staining of human tonsil tissue section using NBP2-61568.

Applications for NFkB p105/p50 Antibody (RM299)

Application

Recommended Usage

Immunohistochemistry

1:100 - 1:200

Immunohistochemistry-Paraffin

1:100-1:200

Simple Western

1:10 - 1:270

Western Blot

1:100 - 1:200

Application Notes

See Simple Western Antibody Database for Simple Western validation: Tested in HeLa Lysate, separated by Size, antibody dilution of 1:10, 1:30, 1:90, 1:270, apparent MW was 56, 126 kDa

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFkB p105/p50

NFkB is a transcription regulator that is activated by various intra and extra cellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth.

Alternate Names

DKFZp686C01211, DNA binding factor KBF1, DNA-binding factor KBF1, EBP-1, KBF1, NF-kappaB, NF-kappa-B, NF-kappabeta, NFKB-p105, NFKB-p50, nuclear factor kappa-B DNA binding subunit, nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151, p105, p50

Gene Symbol

NFKB1

Additional NFkB p105/p50 Products

Product Documents for NFkB p105/p50 Antibody (RM299)

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NFkB p105/p50 Antibody (RM299)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NFkB p105/p50 Antibody (RM299)

There are currently no reviews for this product. Be the first to review NFkB p105/p50 Antibody (RM299) and earn rewards!

Have you used NFkB p105/p50 Antibody (RM299)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image