NFkB p50 (NLS) Inhibitor Peptide Set

Novus Biologicals | Catalog # NBP2-29323

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Key Product Details

Species

Human, Mouse, Rat, Bovine, Canine, Chicken, Xenopus

Applications

Inhibition Activity

Product Specifications

Specificity

The p50 NLS inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.

The control peptide consists of only the PTD sequence

Applications

Functional (Inhibition) (reported in scientific literature (PMID 24384074))

Application Notes

Inhibition of NF-kB p50(NLS) activity. The inhibitor peptide is designed to block p50 nuclear translocation. Optimal peptide concentrations and incubation times vary between model systems and should be determined empirically by users. A 100 uM final concentration may be a useful starting point. Please refer to Lin et al (1995) for additional information about how the inhibitor peptide has been used to block p50 nuclear translocation.

Reactivity Notes

Broad: Peptide sequence is 100% conserved across multiple species.

Inhibitor Content

NF-kB p50 (NLS) Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKVQRKRQKLM (p50 NLS sequence: VQRKRQKLM). Molecular weight: 3529. Antennapedia Control peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361

Scientific Data Images for NFkB p50 (NLS) Inhibitor Peptide Set

Functional (Inhibition): NFkB p50 (NLS) Inhibitor Peptide Set [NBP2-29323]

Functional (Inhibition): NFkB p50 (NLS) Inhibitor Peptide Set [NBP2-29323]

Functional (Inhibition): NFkB p105/p50 Inhibitor Peptide Set [NBP2-29323] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29323) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).

Formulation, Preparation, and Storage

Preparation Method

Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions.
Note: Bring the solution to room temperature and quick spin the tubes before opening the caps. p50 (NLS)
Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKVQRKRQKLM
Add 57 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.

Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.
Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving

Please contact technical support for detailed reconstitution instructions.

Concentration

Lyoph

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFkB p50 (NLS)

The NLS sequence is required for nuclear translocation of NF-kB p50. In stimulated cells, NF-kB is in the cytoplasm in an inactive form and the NLS is masked. The degradation of IkBalpha during NF-kB signaling unmasks the NLS of NF-kB subunits and NF-kB is translocated to the nucleus. The p50 inhibitory peptide contains the p50 NLS sequence (VQRKRQKLM) and blocks the intracellular recognition mechanism for the NLS present on p50. Consequently p50 nuclear translocation is blocked.

Alternate Names

CVID12, EBP-1, KBF1, NFkappaB, NF-kappaB, NF-kappa-B1, NFKB1, NF-kB1, NFKB-p105, NFKB-p50, nuclear factor kappa B subunit 1, p105, P50

Gene Symbol

NFKB1

Additional NFkB p50 (NLS) Products

Product Documents for NFkB p50 (NLS) Inhibitor Peptide Set

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NFkB p50 (NLS) Inhibitor Peptide Set

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

Citations for NFkB p50 (NLS) Inhibitor Peptide Set

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Protocols

View specific protocols for NFkB p50 (NLS) Inhibitor Peptide Set (NBP2-29323):

NFkB p50 (NLS) Inhibitor Peptide Set:
Hazard Information
Chemical Name: Non hazardous products
Chemical Formula: N/A
CAS Number: N/A
EEC-No: N/A

Hazard Identification
None
First Aid Measures
Eye Contact: None
Skin Contact: None
Inhalation: None
Ingestion: None

Accidental Release Measures
This product either does not contain hazardous constituents or the concentration of all chemical constituents are below the regulatory threshold limits described by Occupational Safety Health Administration Hazard Communication Standard 29 CFR 1910.1200 and the European Directive 91/155/EEC. 88/379/EEC, and 67/546/EEC.

Handling and Storage
Exposure Controls / Personal Protection
Other Precautions: None

Physical and Chemical Properties
Form: N/A
Color: N/A
Odor: N/A
Melting Point: N/A
Boiling Temperature: N/A
Density: N/A
Vapor Pressure: N/A
Solubility in Water: N/A
Flash Point: N/A
Explosion limits: N/A
Ignition Temperature: N/A

FAQs for NFkB p50 (NLS) Inhibitor Peptide Set

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  • Q: Please let us know the buffer composition prior to lyophilization. Is it lyophilized from H2O?

    A: Thank you for your inquiry about NBP2-20323, the NFkB p105/p50 Inhibitor Peptide Set. This product is lyophilized from a PBS buffer.

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