NFYB Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-17745

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NFYB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFYB Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NFYB Antibody [NBP3-17745]

Immunocytochemistry/ Immunofluorescence: NFYB Antibody [NBP3-17745]

Immunocytochemistry/Immunofluorescence: NFYB Antibody [NBP3-17745] - Staining of human cell line SiHa shows localization to nucleoplasm.
NFYB Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: NFYB Antibody - BSA Free [NBP3-17745]

Chromatin Immunoprecipitation-exo-Seq: NFYB Antibody - BSA Free [NBP3-17745]

ChIP-Exo-Seq composite graph for Anti-NFYB (NBP3-17745) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for NFYB Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFYB

The protein encoded by the NFYB gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. (provided by RefSeq)

Alternate Names

CAAT box DNA-binding protein subunit B, CBF-A, HAP3CBF-B, NF-YBCCAAT-binding transcription factor subunit A, Nuclear transcription factor Y subunit B, nuclear transcription factor Y subunit beta, nuclear transcription factor Y, beta, Transcription factor NF-Y, B subunit

Gene Symbol

NFYB

Additional NFYB Products

Product Documents for NFYB Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NFYB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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