NHS-LC-Biotin

Key Product Details

Product Description

NHS-LC-Biotin is a membrane permeable amine reactive biotinylation reagent used to attach biotin to primary amines with a 6-atom chain (22.4 angstroms) linker. NHS-LC-Biotin is commonly used to label proteins and antibodies. Reactive in pH 7-9.

This product should be dissolved in DMSO (solubility = 100 mM) or DMF prior to further dilution in aqueous buffers.

Product Specifications for NHS-LC-Biotin

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for NHS-LC-Biotin

The following data is based on the product molecular weight 454.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.20 mL	11.00 mL	22.00 mL
5 mM	0.44 mL	2.20 mL	4.40 mL
10 mM	0.22 mL	1.10 mL	2.20 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Wang An array of 60,000 antibodies for proteome-scale antibody generation and target discovery. Sci.Adv. 2020 PMID: 32195335
• Kamperman Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs. Nat.Commun. 2019 PMID: 31554812

Product Documents for NHS-LC-Biotin