NIH 3T3 CoCl2 treated/Untreated Cell Lysate

SDS-PAGE

NIH 3T3 whole cell lysate is provided as a Western blot positive control. The lysates are provided as a set of treated and untreated samples. Use 10 ul per lane for a standard mini-gel blot (1.0 mg/ml).

Cell

CoCl2 treated/Untreated

NIH 3T3 cells are grown under standard tissue culture conditions until semi-confluent (70-80%). The cells are then treated with or without 100 uM CoCl2 for 4 hours, washed in PBS and directly lysed into 1X Laemmli sample buffer with BME. The lysate is sonicated and tested by Western blot.

The protein lysate is prepared in 1x Laemmli sample buffer with BME. Boiling before loading is not necessary.

1.0 mg/ml

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Store at -20 to -80C. Avoid freeze-thaw cycles.