Ninein Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13657

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ninein Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: EERLTQMRNEYERQCRVLQDQVDELQSELEEYRAQGRVLRLPLKNSPSEEVEANSGGIEPEHGLGSEECNPLNMSIEAELVIEQMKEQHHRDICCLRLELEDKVRHYEKQLDETVVSCKKAQENMKQRHENETHTL

Marker

Centrosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ninein Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Ninein Antibody [NBP2-13657]

Immunocytochemistry/ Immunofluorescence: Ninein Antibody [NBP2-13657]

Immunocytochemistry/Immunofluorescence: Ninein Antibody [NBP2-13657] - Staining of human cell line U-251 MG shows localization to nucleoli and centrosome.
Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657]

Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657]

Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657]

Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657]

Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human tonsil shows moderate cytoplasmic and membranous positivity in germinal center cells.
Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657]

Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657]

Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657]

Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657]

Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human stomach shows moderate cytoplasmic positivity in parietal cells.

Applications for Ninein Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP2-13657 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ninein

Ninein encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.

Alternate Names

Glycogen synthase kinase 3 beta-interacting protein, GSK3B-interacting protein, hNinein, KIAA1565, ninein, ninein (GSK3B interacting protein), ninein centrosomal protein

Gene Symbol

NIN

Additional Ninein Products

Product Documents for Ninein Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Ninein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ninein Antibody - BSA Free

Customer Reviews for Ninein Antibody - BSA Free (1)

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  • Ninein Antibody
    Name: KARTHIKEYAN THIRUGNANAM
    Application: Immunocytochemistry
    Sample Tested: brain microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 10/21/2021
    Red: Centrin2 antibody; Green: Ninein antibody
    Ninein Antibody - BSA Free NBP2-13657

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