NIPBL Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85110

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (99%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NIPBL Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIPBL Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NIPBL Antibody [NBP1-85110]

Immunocytochemistry/ Immunofluorescence: NIPBL Antibody [NBP1-85110]

Immunocytochemistry/Immunofluorescence: NIPBL Antibody [NBP1-85110] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110]

Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110]

Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.
NIPBL Antibody

Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] -

Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] -Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
NIPBL Antibody

Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] -

Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] -Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
NIPBL Antibody

Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] -

Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Applications for NIPBL Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIPBL

NIPBL encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CDLS1, Delangin, DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, FLJ44854, IDN3-B, IDN3CDLS, Nipped-B homolog (Drosophila), nipped-B-like protein, Scc2, SCC2 homolog, sister chromatid cohesion 2 homolog

Gene Symbol

NIPBL

Additional NIPBL Products

Product Documents for NIPBL Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NIPBL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NIPBL Antibody - BSA Free

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Protocols

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FAQs

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