Recombinant Human NKX3.1 His Protein

Novus Biologicals | Catalog # NBP2-23290

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all NKX3.1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-234 of Human NKX3.1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLRVPEP RPGEAKAEGA APPTPSKPLT SFLIQDILRD GAQRQGGRTS SQRQRDPEPE PEPEPEGGRS RAGAQNDQLS TGPRAAPEEA ETLAETEPER HLGSYLLDSE NTSGALPRLP QTPKQPQKRS RAAFSHTQVI ELERKFSHQK YLSAPERAHL AKNLKLTETQ VKIWFQNRRY KTKRKQLSSE LGDLEKHSSL PALKEEAFSR ASLVSVYNSY PYYPYLYCVG SWSPAFW

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

28.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NKX3.1 His Protein

SDS-PAGE: Recombinant Human NKX3.1 His Protein [NBP2-23290]

SDS-PAGE: Recombinant Human NKX3.1 His Protein [NBP2-23290]

SDS-Page: Nkx3.1 Protein [NBP2-23290]

Formulation, Preparation, and Storage

NBP2-23290
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKX3.1

NKX3-1 is a homeobox-containing transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of NKX3-1 is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this. Recombinant human NKX3-1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

NK3 Homeobox 1

Alternate Names

BAPX2, NKX3-1, NKX3A

Gene Symbol

NKX3-1

Additional NKX3.1 Products

Product Documents for Recombinant Human NKX3.1 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human NKX3.1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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