NMNAT-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32464

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LIPAYHRVIMAELATKNSKWVEVDTWESLQKEWKETLKVLRHHQEKLEASDCDHQQNSPTLERPGRKRKWTETQD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NMNAT-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NMNAT-1 Antibody [NBP2-32464]

Immunocytochemistry/ Immunofluorescence: NMNAT-1 Antibody [NBP2-32464]

Immunocytochemistry/Immunofluorescence: NMNAT-1 Antibody [NBP2-32464] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464]

Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464]

Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464] - Staining of human liver shows moderate nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464]

Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464]

Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464] - Staining of human kidney shows strong nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464]

Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464]

Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes.
Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464]

Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464]

Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32464] - Staining of human testis shows moderate nuclear positivity in cells in seminiferious ducts.

Applications for NMNAT-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NMNAT-1

The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).[supplied by OMIM]

Long Name

Nicotinamide mononucleotide adenylyltransferase-1

Alternate Names

NMN adenylyltransferase 1, NMNAT1, PNAT1

Gene Symbol

NMNAT1

Additional NMNAT-1 Products

Product Documents for NMNAT-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NMNAT-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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