Non-Human Primate Angiopoietin-1 Cartridge
R&D Systems | Catalog # SPCKB-NP-011403
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Non-Human Primate Angiopoietin-1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 70 | 70 |
| Mean (pg/mL) | 345 | 15920 | 378 | 17677 |
| Standard Deviation | 16.6 | 778 | 53.9 | 2020 |
| CV% | 4.8 | 4.9 | 14.2 | 11.4 |
Recovery for Non-Human Primate Angiopoietin-1 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=2) | 106 | 105-108 |
Linearity
Samples containing and/or spiked with high concentrations of Angiopoietin-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Non-Human Primate Angiopoietin-1 Cartridge
Non-Human Primate Angiopoietin-1 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Angiopoietin-1
Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system. It signals through the receptor tyrosine kinase Tie-2 and binds a variety of integrins and the extracellular matrix. Angiopoietin-1 induces the development and branching of blood vessels, promotes vascular endothelial cell motility during angiogenesis, and enhances vascular integrity. It can suppress several VEGF-induced effects on the vasculature. Angiopoietin-1 can protect against pulmonary arterial hypertension, reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium, and enhance tumor progression and metastasis.
Additional Angiopoietin-1 Products
Product Documents for Non-Human Primate Angiopoietin-1 Cartridge
Certificate of Analysis
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Product Specific Notices for Non-Human Primate Angiopoietin-1 Cartridge
For research use only
Citations for Non-Human Primate Angiopoietin-1 Cartridge
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