Non-Human Primate P-Selectin/CD62P Cartridge
R&D Systems | Catalog # SPCKB-NP-011335
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Non-Human Primate P-Selectin/CD62P Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 58 | 58 |
| Mean (pg/mL) | 136 | 7673 | 152 | 7536 |
| Standard Deviation | 6.81 | 596 | 14.1 | 625 |
| CV% | 5.0 | 7.8 | 9.3 | 8.3 |
Recovery for Non-Human Primate P-Selectin/CD62P Cartridge
Recovery was not necessary as P-Selectin shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of P-Selectin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Non-Human Primate P-Selectin/CD62P Cartridge
Non-Human Primate P-Selectin/CD62P Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: P-Selectin/CD62P
P-Selectin, also known as CD62P, GMP-140, PADGEM, and LECAM-3, is a transmembrane adhesion protein that is upregulated on activated platelets and vascular endothelial cells. It binds to hematopoietic cell PSGL-1/CD162 with highly clustered O-linked sialyl Lewis-x (sLex ) tetrasaccharides and sulfated tyrosine, as well as to heparin sulfate, Versican, and CD42b/GPIb alpha. P-Selectin mediates the initial interaction of circulating leukocytes with activated endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. It is therefore critical for leukocyte extravasation at sites of inflammation and leukocyte involvement in thrombus formation at sites of vascular injury.
Alternate Names
Gene Symbol
Additional P-Selectin/CD62P Products
Product Documents for Non-Human Primate P-Selectin/CD62P Cartridge
Certificate of Analysis
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Product Specific Notices for Non-Human Primate P-Selectin/CD62P Cartridge
For research use only
Citations for Non-Human Primate P-Selectin/CD62P Cartridge
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