non-muscle heavy chain 10 Myosin Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13221

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all non-muscle heavy chain 10 Myosin Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human non-muscle heavy chain 10 Myosin. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for non-muscle heavy chain 10 Myosin Antibody - BSA Free

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-13221]

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-13221]

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-13221] - Non-transfected (-) and transfected (+) non-muscle heavy chain 10 Myosin-transfected 293T (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody (NBP3-13221) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-13221]

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-13221]

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-13221] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody (NBP3-13221) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
non-muscle heavy chain 10 Myosin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: non-muscle heavy chain 10 Myosin Antibody [NBP3-13221] -

MYH10 antibody detects MYH10 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MYH10 protein stained by MYH10 antibody (NBP3-13221) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for non-muscle heavy chain 10 Myosin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: non-muscle heavy chain 10 Myosin

Myosin II is a hexameric protein that consist of a pair of heavy chain subunits (MHC), a pair of essential light chain subunits (MLC), and a pair of regulatory light chain subunits (RLCs). Non-muscle myosin heavy chain II-B is encoded by the myosin heavy chain 10 gene (MYH10) and non-muscle myosin heavy chain II-A is encoded by the Myosin heavy chain 9 gene (MYH9). Myosin in non-muscle cells is involved in motile cellular processes which include cytokinesis, cell shape, secrection, and capping. Alternate names for Myosin-10 is Myosin heavy chain II-B, cellular myosin heavy chain type B, and NMMHCB.

Long Name

Myosin-10

Alternate Names

MYH10, NMMHC II-b, NMMHC-B, NMMHC-IIB

Gene Symbol

MYH10

Additional non-muscle heavy chain 10 Myosin Products

Product Documents for non-muscle heavy chain 10 Myosin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for non-muscle heavy chain 10 Myosin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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