NOS Activity Assay Kit (Colorimetric)

Novus Biologicals | Catalog # KA1634

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Biological Samples (25 uL)

Sensitivity

0.25 U/L

Assay Range

0.25 to 25 U/L
View all NOS Activity Activity Assays »

Product Specifications

Assay Type

Colorimetric

Kit Type

Assay Kit (Colorimetric)

Species

Mouse, Parasite

Description

Nitric Oxide Synthase Assay Kit is a quantitative colorimetric determination of nitric oxide synthase activity at 540 nm.

Kit Contents for NOS Activity Assay Kit (Colorimetric)

  • Assay Buffer
  • GDH
  • NaOH
  • Reagent A
  • Reagent B
  • Reagent C
  • Reagent D (Dried)
  • Reagent E
  • Standard
  • Substrate
  • ZnSO4

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: NOS Activity

NOS Activity Assay Kit is a method for detecting nitric oxide synthase activity. This is an enzymatic activity assay. Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Nitric Oxide Synthase Activity Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. The procedure is reduced to as short as 40 min.

Additional NOS Activity Products

Product Documents for NOS Activity Assay Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NOS Activity Assay Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations for NOS Activity Assay Kit (Colorimetric)

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FAQs

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