NPE-caged-proton
Tocris Bioscience | Catalog # 3512
Key Product Details
Description
Alternative Names
Product Description
NPE-caged-proton is a 1-(2-nitrophenyl)ethyl caged proton that releases a proton and a sulfate ion upon photolysis at 350 - 355 nm. Generates rapid acidifications (pH jumps) down to pH 2.
Product Specifications for NPE-caged-proton
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 26.92 | 100 | |
| DMSO | 26.92 | 100 |
Preparing Stock Solutions for NPE-caged-proton
The following data is based on the product molecular weight 269.21.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.71 mL | 18.57 mL | 37.15 mL |
| 5 mM | 0.74 mL | 3.71 mL | 7.43 mL |
| 10 mM | 0.37 mL | 1.86 mL | 3.71 mL |
| 50 mM | 0.07 mL | 0.37 mL | 0.74 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 5 publications citing the usage of this product.
- Abbruzzetti Kinetics of proton release after flash photolysis of 1-(2-Nitrophenyl)ethyl sulfate (caged sulfate) in aqueous solution. J.Am.Chem.Soc. 2005 PMID: 15998092
- Peralvarez-Marin Time-resolved infrared spectroscopy of pH-induced aggregation of the Alzheimer Aβ1-28 peptide. J.Mol.Biol. 2008 PMID: 18462754
- Barth and Corrie Characterization of a new caged proton capable of inducing large pH jumps. Biophys.J. 2002 PMID: 12414718
Product Documents for NPE-caged-proton
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NPE-caged-proton
For research use only
Citations for NPE-caged-proton
Customer Reviews for NPE-caged-proton (1)
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Species: HumanVerified Customer | Posted 07/17/2020When photolyzed Caged-proton is supposed to reduce pH; even strong 365nm UV (~1W/cm^2) for 1 second did not show any change in pHBio-Techne ResponseThank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. We suggested using a shorter wavelength for photolysis and offered a replacement vial, but we did not receive a response.
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