Recombinant Human NPL His Protein

Novus Biologicals | Catalog # NBP1-51091

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all NPL Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-320 of Human NPL

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAFPKKKLQG LVAATITPMT ENGEINFSVI GQYVDYLVKE QGVKNIFVNG TTGEGLSLSV SERRQVAEEW VTKGKDKLDQ VIIHVGALSL KESQELAQHA AEIGADGIAV IAPFFLKPWT KDILINFLKE VAAAAPALPF YYYHIPALTG VKIRAEELLD GILDKIPTFQ GLKFSDTDLL DFGQCVDQNR QQQFAFLFGV DEQLLSALVM GATGAVGSTY NYLGKKTNQM LEAFEQKDFS LALNYQFCIQ RFINFVVKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKASR EFTDSAEAKL KSLDFLSFTD LKDGNLEAGS

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

37.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NPL His Protein

SDS-PAGE: Recombinant Human NPL His Protein [NBP1-51091]

SDS-PAGE: Recombinant Human NPL His Protein [NBP1-51091]

SDS-Page: Recombinant Human NPL Protein [NBP1-51091] - 15 % SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-51091
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NPL

NPL, also known as N-acetylneuraminate lyase, is an enzyme that catalyzes the chemical reaction. (N-acetylneuraminate ->N-acetyl-D-mannosamine + pyruvate) This protein belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. It participates in amino sugars metabolism. Recombinant human NPL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

C112, C1orf13MGC61869, chromosome 1 open reading frame 13, DHDPS1, dihydrodipicolinate synthetase homolog 1, EC 4.1.3.3, MGC149582, N-acetylneuraminate lyase, N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase), N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, NAL, NALase, NPL1, Sialate lyase, Sialate-pyruvate lyase, Sialic acid aldolase, Sialic acid lyase

Gene Symbol

NPL

Additional NPL Products

Product Documents for Recombinant Human NPL His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human NPL His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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