Recombinant E. coli NPL His Protein

Novus Biologicals | Catalog # NBP2-22652

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all NPL Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-297 of E.coli NPL

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MATNLRGVMA ALLTPFDQQQ ALDKASLRRL VQFNIQQGID GLYVGGSTGE AFVQSLSERE QVLEIVAEEA KGKIKLIAHV GCVSTAESQQ LAASAKRYGF DAVSAVTPFY YPFSFEEHCD HYRAIIDSAD GLPMVVYNIP ALSGVKLTLD QINTLVTLPG VGALKQTSGD LYQMEQIRRE HPDLVLYNGY DEIFASGLLA GADGGIGSTY NIMGWRYQGI VKALKEGDIQ TAQKLQTECN KVIDLLIKTG VFRGLKTVLH YMDVVSVPLC RKPFGPVDEK YLPELKALAQ QLMQERG

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

34.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli NPL His Protein

SDS-PAGE: Recombinant E. coli NPL His Protein [NBP2-22652]

SDS-PAGE: Recombinant E. coli NPL His Protein [NBP2-22652]

SDS-Page: NPL Protein [NBP2-22652]

Formulation, Preparation, and Storage

NBP2-22652
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NPL

NanA, also known as N-acetylneuraminate lyase, belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. This protein was inhibited by reduction with NaBH4 in the presence of the substrate, indicating that it belongs to the Schiff-base-forming Class I aldolases. NanA was strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, and also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde. Recombinant E.coli nanA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

C112, C1orf13MGC61869, chromosome 1 open reading frame 13, DHDPS1, dihydrodipicolinate synthetase homolog 1, EC 4.1.3.3, MGC149582, N-acetylneuraminate lyase, N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase), N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, NAL, NALase, NPL1, Sialate lyase, Sialate-pyruvate lyase, Sialic acid aldolase, Sialic acid lyase

Gene Symbol

NPL

Additional NPL Products

Product Documents for Recombinant E. coli NPL His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant E. coli NPL His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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