NR12S
Tocris Bioscience | Catalog # 7509
Key Product Details
Description
Wavelength
Product Description
Key information: NR12S is a fluorogenic lipid membrane dye that stains the outer leaflet of lipid vesicles and cell membranes. Suitable for live cell imaging.Used for: monitoring cholesterol and lipid order selectively at the outer leaflet of cell membranes.
Application: fluorescence microscopy.
Properties and Photophysical Data: NR12S is a conjugation of the Nile Red fluorophore with a long alkyl chain and a zwitterionic group, allowing strong interactions with the lipid membranes. Excitation and emission maxima (λ) are 554 nm and 627 nm in DMSO, respectively; quantum yield = 0.55.
Optical Data
| Emission Color | Orange |
| λabs | 554 nm |
| λem | 627 nm |
| Quantum Yield (φ) | 0.55 |
| Closest Laser line | 532 nm |
| Application | Monitoring of Lipid Dynamics in Living Cells, Fluorescence Microscopy |
| Alternative For | Nile Red |
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Product Specifications for NR12S
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.96 | 10 |
Preparing Stock Solutions for NR12S
The following data is based on the product molecular weight 695.96.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 14.37 mL | 71.84 mL | 143.69 mL |
| 0.5 mM | 2.87 mL | 14.37 mL | 28.74 mL |
| 1 mM | 1.44 mL | 7.18 mL | 14.37 mL |
| 5 mM | 0.29 mL | 1.44 mL | 2.87 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 8 publications citing the usage of this product.
- Kucherak Switchable nile red-based probe for cholesterol and lipid order at the outer leaflet of biomembranes. J.Am.Chem.Soc. 2010 PMID: 20225874
Product Documents for NR12S
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NR12S
For research use only
Citations for NR12S
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