Nucleic Acid Dye Green I

Catalog # Availability Size / Price Qty
Nucleic Acid Dye Green I | CAS No. 2225748-05-8 | Fluorescent DNA Stains
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Description: High affinity double-stranded DNA (dsDNA) probe; used in RT-LAMP and qPCR

Chemical Name: 2-((2-((3-(Dimethylamino)propyl)(propyl)amino)-1-phenylquinolin-4(1H)-ylidene)methyl)-3-methylbenzo[d]thiazol-3-ium chloride

Purity: ≥95%

Product Details

Biological Activity

Nucleic Acid Dye Green I is an asymmetric cyanine dye that specifically binds to dsDNA. Frequently used dye in qPCR and is further used as a stain for in-gel visualization of DNA. Also used in the detection of amplification products in Loop-Mediated Isothermal Amplification (LAMP) assays; this method has been used for the detection of SARS-CoV-2 virus. When bound to dsDNA the fluorescence intensity is 1000-fold greater than when in the unbound form. Does not require washing or de-staining steps. Compatible with all commonly used fluorescence detection systems. Excitation maximum = 495 nm; emission maximum = 520 nm.

This product is sold as a DMSO stock.

Usage Information

  • Supplied as a 10,000x concentrate in DMSO.
  • Soluble in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), TBE, or TAE buffer.
  • Verify that the pH of the staining solution, at the temperature used for staining, is between 7.5 and 8.0 (preferably pH 8.0).
  • Use diluted samples within 24 hours to ensure maximal sensitivity.

Optical Data for Nucleic Acid Dye Green I

Nucleic Acid Dye Green I Dye Spectra
Emission Color Green
λabs 495 nm
λem 520 nm
Application Real Time PCR, Nucleic Acid Staining, RT-LAMP
Alternative For SYBR® Green I

Technical Data

Soluble in DMSO (supplied as a 10,000x concentrate in DMSO)
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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