Nucleic Acid Dye Green I

Tocris Bioscience | Catalog # 7246

High affinity double-stranded DNA (dsDNA) probe; used in RT-LAMP and qPCR
Tocris Bioscience
Discontinued Product
7246 has been discontinued. View all Fluorescent DNA Stains products.

Key Product Details

Description

High affinity double-stranded DNA (dsDNA) probe; used in RT-LAMP and qPCR

Wavelength

Excitation = 495 nm, Emission = 520 nm
View all Fluorescent DNA Stains »

Product Description

Nucleic Acid Dye Green I is an asymmetric cyanine dye that specifically binds to dsDNA. Frequently used dye in qPCR and is further used as a stain for in-gel visualization of DNA. Also used in the detection of amplification products in Loop-Mediated Isothermal Amplification (LAMP) assays; this method has been used for the detection of SARS-CoV-2 virus. When bound to dsDNA the fluorescence intensity is 1000-fold greater than when in the unbound form. Does not require washing or de-staining steps. Compatible with all commonly used fluorescence detection systems. Excitation maximum = 495 nm; emission maximum = 520 nm.

This product is sold as a DMSO stock.

Usage Information

  • Supplied as a 10,000x concentrate in DMSO.
  • Soluble in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), TBE, or TAE buffer.
  • Verify that the pH of the staining solution, at the temperature used for staining, is between 7.5 and 8.0 (preferably pH 8.0).
  • Use diluted samples within 24 hours to ensure maximal sensitivity.

Optical Data

Nucleic Acid Dye Green I Dye Spectra
Emission Color Green
λabs 495 nm
λem 520 nm
Application Real Time PCR, Nucleic Acid Staining, RT-LAMP
Alternative For SYBR® Green I

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Product Specifications for Nucleic Acid Dye Green I

Molecular Weight

545.19

Formula

C32H37ClN4S

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-((2-((3-(Dimethylamino)propyl)(propyl)amino)-1-phenylquinolin-4(1H)-ylidene)methyl)-3-methylbenzo[d]thiazol-3-ium chloride

CAS Number

2225748-05-8

PubChem ID

131742173

InChI Key

IHHRNZWXTFDDLM-UHFFFAOYSA-M

SMILES

CCCN(C(N(C1=C/2C=CC=C1)C3=CC=CC=C3)=CC2=C\C4=[N+](C)C5=CC=CC=C5S4)CCCN(C)C.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble in DMSO (supplied as a 10,000x concentrate in DMSO)

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Product Documents for Nucleic Acid Dye Green I

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Nucleic Acid Dye Green I

For research use only

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